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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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CXCL10 / IP‑10 Antibody LS‑C104828
Note: This antibody replaces LS-B3295
IP-10 antibody LS-C104828 is an unconjugated rabbit polyclonal antibody to rat IP-10 (CXCL10). Validated for ELISA and WB.
Catalog
Size
Price
LS-C104828-50
50 µg
$285
LS-C104828-100
100 µg
$325
Description
IP-10 antibody LS-C104828 is an unconjugated rabbit polyclonal antibody to rat IP-10 (CXCL10). Validated for ELISA and WB.
Target
Rat CXCL10 / IP-10
Host
Rabbit
Reactivity
Rat (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated. Also available conjugated with Biotin.
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • Western blot (0.1 - 0.2 µg/ml)
  • ELISA (0.5 - 2 µg/ml)
  • (applications tested for the base form of this product only)
Immunogen
CXCL10 / IP-10 antibody was raised against e.coli derived recombinant Rat IP-10.
Specificity
Rat IP-10
Usage
Immunohistochemistry: This antibody stained colchicine injected rat brain (including the caudate putamen) tissue. The primary antibody was incubated at 0.25 ug/ml overnight at 4°C. This was followed by a peroxidase conjugated secondary antibody and then a fluorescein Tyramide Signal Amplification (TSA) reagent. Optimal concentrations and conditions may vary. Information and photo are courtesy of the Tissue Profiling group, SciLifeLab Stockholm. Neutralization: To yield one-half maximal inhibition [ND] of the biological activity of Rat IP-10 (100 ng/ml), a concentration of 5-10 ug/ml of this antibody is required. ELISA: To detect Rat IP-10 by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5-2 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Rat IP-10 (LS-C104821) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant Rat IP-10. Western Blot: To detect Rat IP-10 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant Rat IP-10 is 1.5-3 ng/lane, under either reducing or non-reducing conditions.
Presentation
Lyophilized from PBS, pH 7.2
Reconstitution
Sterile water
Storage
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
About CXCL10 / IP-10
P02778 NM_001565 NP_001556.2

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Western Blot (reducing) of IP-10 / CXCL10 antibody. This image was taken for the unconjugated form of this product. Other forms have not been tested.
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Western Blot (reducing) of IP-10 / CXCL10 antibody. This image was taken for the unconjugated form of this product. Other forms have not been tested.
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Images

Western blot

Western Blot (reducing) of IP-10 / CXCL10 antibody. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Western Blot (reducing) of IP-10 / CXCL10 antibody. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Western Blot (non-reducing) of IP-10 / CXCL10 antibody. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Western Blot (non-reducing) of IP-10 / CXCL10 antibody. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Enzyme-Linked Immunosorbent Assay

Sandwich ELISA of IP-10 / CXCL10 antibody. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Sandwich ELISA of IP-10 / CXCL10 antibody. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Western Blot (reducing) of IP-10 / CXCL10 antibody. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Western Blot (reducing) of IP-10 / CXCL10 antibody. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Western Blot (non-reducing) of IP-10 / CXCL10 antibody. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Western Blot (non-reducing) of IP-10 / CXCL10 antibody. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Enzyme-Linked Immunosorbent Assay

Sandwich ELISA of IP-10 / CXCL10 antibody. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Sandwich ELISA of IP-10 / CXCL10 antibody. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Western Blot (reducing) of IP-10 / CXCL10 antibody. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Western Blot (reducing) of IP-10 / CXCL10 antibody. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Western Blot (non-reducing) of IP-10 / CXCL10 antibody. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Western Blot (non-reducing) of IP-10 / CXCL10 antibody. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Enzyme-Linked Immunosorbent Assay

Sandwich ELISA of IP-10 / CXCL10 antibody. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Sandwich ELISA of IP-10 / CXCL10 antibody. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Western Blot (reducing) of IP-10 / CXCL10 antibody. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Western Blot (reducing) of IP-10 / CXCL10 antibody. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Western Blot (non-reducing) of IP-10 / CXCL10 antibody. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Western Blot (non-reducing) of IP-10 / CXCL10 antibody. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Enzyme-Linked Immunosorbent Assay

Sandwich ELISA of IP-10 / CXCL10 antibody. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Sandwich ELISA of IP-10 / CXCL10 antibody. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Requested From: United States
Date Requested: 11/17/2018
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