Target
Human CTNNB1 / Beta Catenin
Synonyms
CTNNB1 | Beta-catenin | Catenin beta-1 | CTNNB | Beta Catenin
Reactivity
Human
(tested or 100% immunogen sequence identity)
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Immunogen
Synthetic peptide corresponding to amino acid residues around tyrosine 142 of human b-Catenin (KLH).
Specificity
Recognizes a 92kD protein corresponding to the molecular mass of b-Catenin on SDS-PAGE immunoblots of Hct116 src transformed cells treated with pervanadate, but not in control cells. Similar results were observed in other pervanadate-treated cell lines, such as A431 and human endothelial cells. Species sequence homology: mouse and rat. Sequence homology: one amino acid difference from a sequence around tyrosine 133 of human g-Catenin.
Applications
- Western blot (1:500)
- ELISA (1:2000)
Usage
Suitable for use in ELISA, Western Blot or for antigen applications in immunological protocols. Western Blot: 1:500. Membrane was incubated with diluted antibody in 5% non-fat milk, PBS, 0.04% Tween 20 for 1 hour at RT. ELISA: 1:2000.
Presentation
PBS, 1 mg/ml BSA, 0.05% Sodium Azide, 50% Glycerol
Storage
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About CTNNB1 / Beta Catenin