Target
Human CTNNB1 / Beta Catenin
Synonyms
CTNNB1 | Beta-catenin | Catenin beta-1 | CTNNB | Beta Catenin
Reactivity
Human
(tested or 100% immunogen sequence identity)
Conjugations
Unconjugated
Purification
Affinity purified
Immunogen
Recombinant human beta-catenin (~88kD)
Specificity
Recognizes an epitope in the carboxy terminus, amino acids 758-771, of human beta-catenin.
Applications
- IHC
- IHC - Paraffin (10 µg/ml)
- ICC
- Western blot
- Immunoprecipitation
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Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
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Usage
Immunohistochemistry: LS-B3110 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3110 was determined to be 10 ug/ml.
Presentation
PBS, pH 7.6. No stabilizing proteins, preservatives, no glycerol added.
Storage
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About CTNNB1 / Beta Catenin