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Antibodies > CRK > LS-C808519
 
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Catalog Number Size Price
LS-C808519-100 100 µl (1 mg/ml) $379 
LS-C808519-200 200 µl (1 mg/ml) $421 
CRK Antibody - Staining COS7 cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 min at 25°C. The primary antibody was diluted at 1:200 and incubated with the sample for 1 hour at 37°C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.
CRK Antibody - Staining HuvEc cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% saponin prior to blocking in 10% serum for 45 min at 37°C. The primary antibody was diluted 1/400 and incubated with the sample for 1 hour at 37°C. A Alexa Fluor 594 conjugated goat polyclonal to rabbit IgG (H+L), diluted 1/600 was used as secondary antibody.
CRK Antibody - Western blot analysis of CrkII phosphorylation expression in COS7 whole cells lysates. The lane on the left is treated with the antigen-specific peptide.
CRK Antibody - Western blot analysis of Phospho-CrkII (Tyr221) expression in various lysates
CRK Antibody - Staining COS7 cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 min at 25°C. The primary antibody was diluted at 1:200 and incubated with the sample for 1 hour at 37°C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.
CRK Antibody - Staining HuvEc cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% saponin prior to blocking in 10% serum for 45 min at 37°C. The primary antibody was diluted 1/400 and incubated with the sample for 1 hour at 37°C. A Alexa Fluor 594 conjugated goat polyclonal to rabbit IgG (H+L), diluted 1/600 was used as secondary antibody.
CRK Antibody - Western blot analysis of CrkII phosphorylation expression in COS7 whole cells lysates. The lane on the left is treated with the antigen-specific peptide.
CRK Antibody - Western blot analysis of Phospho-CrkII (Tyr221) expression in various lysates
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Polyclonal Rabbit anti‑Human CRK Antibody (phospho‑Tyr221, IF, WB) LS‑C808519

Polyclonal Rabbit anti‑Human CRK Antibody (phospho‑Tyr221, IF, WB) LS‑C808519

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Antibody:
CRK Rabbit anti-Human Polyclonal (pTyr221) Antibody
Application:
IF, WB, Peptide-ELISA
Reactivity:
Human, Monkey, Mouse, Rat
Format:
Unconjugated, Unmodified
Price
Catalog Number
$379
LS-C808519-100
Toll Free North America
206-374-1102
For Research Use Only
Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:
CRK Rabbit anti-Human Polyclonal (pTyr221) Antibody
Application:
IF, WB, Peptide-ELISA
Reactivity:
Human, Monkey, Mouse, Rat
Format:
Unconjugated, Unmodified

Specifications

Description
CRK antibody LS-C808519 is an unconjugated rabbit polyclonal antibody to CRK (pTyr221) from human. It is reactive with human, mouse, rat and other species. Validated for IF, Peptide-ELISA and WB.
Target
Human CRK
Synonyms
CRK | Adapter molecule crk | CRKII | Proto-oncogene c-Crk | p38
Host
Rabbit
Reactivity
Human, Monkey, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.
Modifications
Unmodified
Immunogen
A synthesized peptide derived from human CrkII around the phosphorylation site of Tyrosine 221.
Epitope
pTyr221
Specificity
Phospho-CrkII (Tyr221) Antibody detects endogenous levels of CrkII only when phosphorylated at Tyrosine 221.
Applications
  • Immunofluorescence (1:100 - 1:500)
  • Western blot (1:500 - 1:2000)
  • Peptide Enzyme-Linked Immunosorbent Assay (1:20000 - 1:40000)
Usage
For western blots: incubate membrane with diluted antibody overnight in 5% w/v milk , 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking.
Presentation
PBS, pH 7.4, 0.02% Sodium Azide, 50% Glycerol
Storage
Upon receipt, store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About CRK
P46108 NM_016823 NP_058431.2

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/30/2024
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