Target
Human CPE / Carboxypeptidase E
Synonyms
CPE | Carboxypeptidase E | CPH | Carboxypeptidase H | Enkephalin convertase
Reactivity
Human
(tested or 100% immunogen sequence identity)
Conjugations
Unconjugated
Purification
Affinity purified
Immunogen
NS0-derived recombinant human Carboxypeptidase E (aa42-453).
Specificity
Recognizes human Carboxypeptidase E. Cross-reactivity: Shows less than 1% cross-reactivity with rhCPA1, rmCPA4 and rhCPB1 in ELISA and Western Blot.
Applications
- Western blot (0.1 - 0.2 µg/ml)
- Immunoprecipitation
- Flow Cytometry
- ELISA (0.1 - 0.2 µg/ml)
Usage
Suitable for use in ELISA, Western Blot, Immunoprecipitation and Flow Cytometry. ELISA: 0.1-0.2 ug/ml. Detection limit for rhCPE is ~1 ng/well. Western Blot: 0.1-0.2 ug/ml. Detection limit for rhCPE is ~5 ng/lane under reducing and non-reducing conditions. Flow Cytometry: (A172 cells): For intracellular staining cells must first be fixed and permeabilized using 4% paraformaldehyde and 0.1% saponin in PBS. Dilute this antibody to 25 ug/mL and add 10uL to 1-5x10^5 cells in a total reaction volume not exceeding 200ul. ELISA: 0.5-1 ug/ml with the appropriate secondary reagents. The detection limit is ~1 ng/well. Immunoprecipitation: Used to immunoprecipitate rhCPE from conditioned media of transfected NSO cells.
Presentation
Lyophilized from PBS, 5% Trehalose
Reconstitution
Reconstitute in sterile PBS.
Storage
Store at -20°C. After reconstitution, aliquot and store at -20°C. Avoid freeze/thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About CPE / Carboxypeptidase E