(tested or 100% immunogen sequence identity)
Immunohistochemistry: LS-B1100 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B1100 was determined to be 5 ug/ml. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
Store at 4°C or -20°C. Avoid freeze-thaw cycles.
This antibody carries the LSBio 100% Guarantee.