Rat CNTF

Rabbit

Rat (tested or 100% immunogen sequence identity)

Polyclonal

Unconjugated. Also available conjugated with Biotin.

Immunoaffinity purified

Unmodified

E.coli derived recombinant Rat CNTF.

Rat CNTF

• Western blot (0.1 - 0.2 µg/ml)
• ELISA (0.5 - 2 µg/ml)

Immunohistochemistry: This antibody stained colchicine injected rat brain (including the globus pallidus region) tissue. The primary antibody was incubated at 0.25 mg/ml overnight at 4°C. This was followed by a peroxidase conjugated secondary antibody and then a fluorescein Tyramide Signal Amplification (TSA) reagent. Optimal concentrations and conditions may vary. Information and photo are courtesy of the Tissue Profiling group, SciLifeLab Stockholm. Neutralization: To yield one-half maximal inhibition [ND] of the biological activity of Rat CNTF (0.15 ng/ml), a concentration of 0.02-0.05 ug/ml of this antibody is required. ELISA: To detect Rat CNTF by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5-2 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Rat CNTF (LS-C104736) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant Rat CNTF. Western Blot: To detect Rat CNTF by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant Rat CNTF is 1.5-3 ng/lane, under either reducing or non-reducing conditions.

Lyophilized from PBS, pH 7.2

Reconstitute with sterile water

Store Lyophilized at room temperature up to 1 month; Reconstituted for up to 2 weeks at 2°C to 8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P26441 NCBI: NM_000614 NP_000605.1