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Catalog Number Size Price
LS-C313426-10 10 µg $318 
LS-C313426-100 100 µg (0.5 mg/ml) $470 
CNTF Antibody - Immunohistochemistry - Anti-CNTF Picoband Antibody
CNTF Antibody - Immunohistochemistry - Anti-CNTF Picoband Antibody
CNTF Antibody - Western blot analysis of CNTF using anti-CNTF antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: mouse brain tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CNTF antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for CNTF at approximately 29KD. The expected band size for CNTF is at 23KD.
CNTF Antibody - Western blot - Anti-CNTF Picoband Antibody
CNTF Antibody - Immunohistochemistry - Anti-CNTF Picoband Antibody
CNTF Antibody - Immunohistochemistry - Anti-CNTF Picoband Antibody
CNTF Antibody - Western blot analysis of CNTF using anti-CNTF antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: mouse brain tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CNTF antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for CNTF at approximately 29KD. The expected band size for CNTF is at 23KD.
CNTF Antibody - Western blot - Anti-CNTF Picoband Antibody
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Polyclonal Rabbit anti‑Mouse CNTF Antibody (aa2‑198, IHC, WB) LS‑C313426

Polyclonal Rabbit anti‑Mouse CNTF Antibody (aa2‑198, IHC, WB) LS‑C313426

Antibody:
CNTF Rabbit anti-Mouse Polyclonal (aa2-198) Antibody
Application:
IHC, IHC-P, WB
Reactivity:
Mouse, Rat
Format:
Unconjugated, Unmodified
Price
Catalog Number
$318
LS-C313426-10
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
CNTF Rabbit anti-Mouse Polyclonal (aa2-198) Antibody
Application:
IHC, IHC-P, WB
Reactivity:
Mouse, Rat
Format:
Unconjugated, Unmodified

Specifications

Description
CNTF antibody LS-C313426 is an unconjugated rabbit polyclonal antibody to CNTF (aa2-198) from mouse. It is reactive with mouse and rat. Validated for IHC and WB.
Target
Mouse CNTF
Synonyms
CNTF | Ciliary neurotrophic factor | HCNTF
Host
Rabbit
Reactivity
Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunogen affinity purified
Modifications
Unmodified
Immunogen
E.coli-derived mouse CNTF recombinant protein (Position: A2-M198). Mouse CNTF shares 83% and 95% amino acid (aa) sequences identity with human and rat CNTF, respectively.
Epitope
aa2-198
Specificity
Nervous system.
Applications
  • IHC
  • IHC - Paraffin
  • Western blot
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Presentation
Lyophilized from 0.2mg Na2HPO4, 5mg BSA, 0.9mg NaCl, 0.05mg sodium azide.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500µg/ml.
Storage
At -20°C for 1 year. After reconstitution, at 4°C for 1 month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About CNTF
P26441 NM_000614 NP_000605.1

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 4/17/2024