(tested or 100% immunogen sequence identity)
Human, Mouse, Dog (at least 90% immunogen sequence identity)
- Western blot (1:1000 - 1:5000)
- ELISA (1:10000 - 1:50000)
CLCN1 / CLC-1 antibody was raised against an 18 AA Peptide sequence near the C-terminus of rat CLC-1 (1). The peptide was coupled to KLH.
The 18 AA rat control peptide 100% conserved in mouse, rat and human CLC-1. No significant sequence homology is detected with other CLCs or other proteins. Because of the low MW (<3kD) of the control peptide, it is not recommended for Western. The control peptide is available to confirm specificity of antibodies.
Suitable for use in ELISA, Western Blot. Western Blot: 1:1000-1:5000 for neat serum and 1-10 ug/ml for affinity pure antibody using ECL. ELISA:1:10000-1:50000 for neat serum and 0.5-1 ug/ml for affinity purified. Control peptide can be used to coat ELISA plates at 1 ug/ml.
PBS, 0.05% sodium azide.
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
For research use only.
This antibody carries the LSBio 100% Guarantee.