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Catalog Number Size Price
LS-C784211-100 100 µg $511 
CHEK2 / CHK2 Antibody - IHC of paraffin-embedded Adenocarcinoma of ovary tissue using anti-CHEK2 mouse monoclonal antibody. (Dilution 1:50).
CHEK2 / CHK2 Antibody - IHC of paraffin-embedded pancreas tissue using anti-CHEK2 mouse monoclonal antibody. (Dilution 1:50).
CHEK2 / CHK2 Antibody - IHC of paraffin-embedded colon tissue using anti-CHEK2 mouse monoclonal antibody. (Dilution 1:50).
CHEK2 / CHK2 Antibody - IHC of paraffin-embedded liver tissue using anti-CHEK2 mouse monoclonal antibody. (Dilution 1:50).
CHEK2 / CHK2 Antibody - Western blot of extracts (35 ug) from 9 different cell lines by using anti-CHEK2 monoclonal antibody.
CHEK2 / CHK2 Antibody - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CHEK2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CHEK2.
CHEK2 / CHK2 Antibody - Immunoprecipitation(IP) of CHEK2 by using monoclonal anti-CHEK2 antibodies (Negative control: IP without adding anti-CHEK2 antibody.). For each experiment, 500ul of DDK tagged CHEK2 overexpression lysates (at 1:5 dilution with HEK293T lysate), 2 ug of anti-CHEK2 antibody and 20ul (0.1 mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.
CHEK2 / CHK2 Antibody - Flow cytometry of Jurkat cells, using anti-CHEK2 antibody (Red), compared to a nonspecific negative control antibody (Blue).
CHEK2 / CHK2 Antibody - HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-CHEK2 antibody, and then analyzed by flow cytometry.
CHEK2 / CHK2 Antibody - IHC of paraffin-embedded Adenocarcinoma of ovary tissue using anti-CHEK2 mouse monoclonal antibody. (Dilution 1:50).
CHEK2 / CHK2 Antibody - IHC of paraffin-embedded pancreas tissue using anti-CHEK2 mouse monoclonal antibody. (Dilution 1:50).
CHEK2 / CHK2 Antibody - IHC of paraffin-embedded colon tissue using anti-CHEK2 mouse monoclonal antibody. (Dilution 1:50).
CHEK2 / CHK2 Antibody - IHC of paraffin-embedded liver tissue using anti-CHEK2 mouse monoclonal antibody. (Dilution 1:50).
CHEK2 / CHK2 Antibody - Western blot of extracts (35 ug) from 9 different cell lines by using anti-CHEK2 monoclonal antibody.
CHEK2 / CHK2 Antibody - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CHEK2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CHEK2.
CHEK2 / CHK2 Antibody - Immunoprecipitation(IP) of CHEK2 by using monoclonal anti-CHEK2 antibodies (Negative control: IP without adding anti-CHEK2 antibody.). For each experiment, 500ul of DDK tagged CHEK2 overexpression lysates (at 1:5 dilution with HEK293T lysate), 2 ug of anti-CHEK2 antibody and 20ul (0.1 mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.
CHEK2 / CHK2 Antibody - Flow cytometry of Jurkat cells, using anti-CHEK2 antibody (Red), compared to a nonspecific negative control antibody (Blue).
CHEK2 / CHK2 Antibody - HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-CHEK2 antibody, and then analyzed by flow cytometry.
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Monoclonal Mouse anti‑Human CHEK2 / CHK2 Antibody (clone OTI2H5, Carrier‑free, IHC, WB) LS‑C784211

Monoclonal Mouse anti‑Human CHEK2 / CHK2 Antibody (clone OTI2H5, Carrier‑free, IHC, WB) LS‑C784211

Antibody:
CHEK2 / CHK2 Mouse anti-Human Monoclonal (Carrier-free) (OTI2H5) Antibody
Application:
IHC, WB, IP, Flo
Reactivity:
Human, Dog
Format:
Unconjugated, Carrier-free
Other formats:
Price
Catalog Number
$511
LS-C784211-100
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
CHEK2 / CHK2 Mouse anti-Human Monoclonal (Carrier-free) (OTI2H5) Antibody
Application:
IHC, WB, IP, Flo
Reactivity:
Human, Dog
Format:
Unconjugated, Carrier-free
Other formats:

Specifications

Description
CHK2 antibody LS-C784211 is an unconjugated mouse monoclonal antibody to CHK2 (CHEK2) from human. It is reactive with human and dog. Validated for Flow, IHC, IP and WB.
Target
Human CHEK2 / CHK2
Synonyms
CHEK2 | BA444G7 | Cds1 homolog | Checkpoint kinase 2 | Checkpoint-like protein CHK2 | CHK2 checkpoint homolog | CHK2 | HCds1 | LFS2 | RAD53 | Rad53 homolog | HCHK2 | HuCds1 | PP1425
Host
Mouse
Reactivity
Human, Dog (tested or 100% immunogen sequence identity)
Clonality
IgG2b Monoclonal
Clone
OTI2H5
Conjugations
Unconjugated
Purification
Purified from ascites.
Modifications
Carrier-free. Also available Unmodified.
Immunogen
Full length human recombinant protein of human CHEK2 (NP_009125) produced in HEK293T cell.
Applications
  • IHC (1:150)
  • Western blot (1:1000 - 1:2000)
  • Immunoprecipitation (4 µg/ml)
  • Flow Cytometry (1:100)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
Applications should be user optimized.
Presentation
PBS, pH 7.3, 8% Trehalose
Reconstitution
Reconstitute with PBS pH 7.3. To use this carrier-free antibody for conjugation experiment, we strongly recommend you to perform another round of desalting process.
Storage
Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About CHEK2 / CHK2
O96017 NM_007194 NP_009125.1

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 6/24/2024