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Immunohistochemistry Services

Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

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Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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CHEK2 / CHK2

checkpoint kinase 2

Serine/threonine-protein kinase which is required for checkpoint-mediated cell cycle arrest, activation of DNA repair and apoptosis in response to the presence of DNA double-strand breaks. May also negatively regulate cell cycle progression during unperturbed cell cycles. Following activation, phosphorylates numerous effectors preferentially at the consensus sequence [L-X-R-X-X-S/T]. Regulates cell cycle checkpoint arrest through phosphorylation of CDC25A, CDC25B and CDC25C, inhibiting their activity. Inhibition of CDC25 phosphatase activity leads to increased inhibitory tyrosine phosphorylation of CDK-cyclin complexes and blocks cell cycle progression. May also phosphorylate NEK6 which is involved in G2/M cell cycle arrest. Regulates DNA repair through phosphorylation of BRCA2, enhancing the association of RAD51 with chromatin which promotes DNA repair by homologous recombination. Also stimulates the transcription of genes involved in DNA repair (including BRCA2) through the phosphorylation and activation of the transcription factor FOXM1. Regulates apoptosis through the phosphorylation of p53/TP53, MDM4 and PML. Phosphorylation of p53/TP53 at 'Ser-20' by CHEK2 may alleviate inhibition by MDM2, leading to accumulation of active p53/TP53. Phosphorylation of MDM4 may also reduce degradation of p53/TP53. Also controls the transcription of pro-apoptotic genes through phosphorylation of the transcription factor E2F1. Tumor suppressor, it may also have a DNA damage-independent function in mitotic spindle assembly by phosphorylating BRCA1. Its absence may be a cause of the chromosomal instability observed in some cancer cells.

Gene Name: checkpoint kinase 2
Family/Subfamily: Protein Kinase , CDS1
Synonyms: CHEK2, BA444G7, Cds1 homolog, Checkpoint kinase 2, Checkpoint-like protein CHK2, CHK2 checkpoint homolog, CHK2, HCds1, LFS2, RAD53, Rad53 homolog, HCHK2, HuCds1, PP1425
Target Sequences: NM_007194 NP_009125.1 O96017

Filtered by: None
Target
CHEK2 / CHK2 (165)
Reactivity
Human (160)
Mouse (69)
Rat (54)
Bat (1)
Bovine (9)
Chicken (1)
Chimpanzee (6)
Dog (16)
Gibbon (3)
Guinea pig (1)
Horse (4)
Monkey (23)
Non-Human Primate (1)
Pig (9)
Rabbit (4)
Sheep (1)
Xenopus (1)
Zebrafish (1)
Application
IHC (70)
IHC-P (33)
WB (157)
Flo (15)
ELISA (38)
ICC (13)
IF (38)
IP (39)
Peptide-ELISA (7)
Host
rabbit (120)
mouse (45)
Isotype
IgG (79)
IgG1 (16)
IgG1,k (10)
IgG2a (4)
IgG2a,k (3)
IgG2b (8)
Clonality
monoclonal mc (43)
polyclonal pc (122)
Clone
1C12B8 (2)
2CHK01 (2)
3E10 (1)
4B7 (1)
4F10 (1)
73C175.1.1 (2)
8F12 (1)
DCS-270 (2)
DCS-273 (2)
OTI10G7 (2)
OTI2F4 (2)
OTI2H5 (2)
OTI4C10 (2)
OTI5C4 (2)
OTI5C7 (2)
OTI8D11 (2)
Format
AP Conjugated (4)
APC (1)
APC Conjugated (5)
Azide-free (2)
Biotin Conjugated (8)
Carrier-free (7)
Cy3 Conjugated (1)
Cy7 Conjugated (1)
FITC Conjugated (8)
HRP Conjugated (8)
Magnetic beads Conjugated (1)
PE Conjugated (5)
Sepharose Conjugated (1)
Unconjugated (123)
Epitope
N-Terminus (12)
aa300-540 (8)
pSer516 (8)
pThr68 (7)
pThr387 (6)
C-Terminus (5)
330-410 aa (4)
pThr383 (4)
10-90 aa (2)
460-540 aa (2)
aa427-441 (2)
aa475-525 (2)
pThr432 (2)
Internal (1)
Met381 (1)
Ser379 (1)
Ser62 (1)
aa1-50 (1)
aa133-148 (1)
aa2-18 (1)
aa35-84 (1)
aa356-405 (1)
aa361-410 (1)
aa465-498 (1)
aa486-535 (1)
pSer19 (1)
pSer33/35 (1)
pSer33/Ser35 (1)
Publications
No (165)
Showing 1-25 of 165 results

IHC-plus™ CHEK2 / CHK2 Antibody LS-B1348
Rabbit Polyclonal (IgG) to Human CHEK2 / CHK2
Mouse, Rat, Human
ELISA, ICC, IF, IHC, IHC-P, WB
Unconjugated
0.05mg 1mg/ml  $425.00
Anti-CHEK2 / CHK2 antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Immunofluorescence of Chk2 in Jurkat cells with Chk2 antibody at 5 µg/mLRed: Chk2 Antibody  Blue: DAPI staining

CHEK2 / CHK2 Antibody (phospho-Thr68) LS-C177890
Rabbit Polyclonal to Human CHEK2 / CHK2
Mouse, Rat, Human
IHC, IHC-P, WB
Unconjugated
100µl 1mg/ml  $345.00
IHC of p-Chk2 (T68) pAb in paraffin-embedded human breast carcinoma tissue.
Western blot of p-Chk2 (T68) pAb in extracts from NIH/3T3 cells treated with UV.

IHC-plus™ CHEK2 / CHK2 Antibody (clone 4B7) LS-B6212
Mouse Monoclonal [clone 4B7] (IgG2a,k) to Human CHEK2 / CHK2
Human
ELISA, IF, IHC, IHC-P, WB
Unconjugated
50µg  $495.00
Anti-CHEK2 / CHK2 antibody IHC of human colon. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 3 ug/ml.
Immunofluorescence of monoclonal antibody to CHEK2 on HeLa cell. [antibody concentration 10 ug/ml]

CHEK2 / CHK2 Antibody LS-C123232
Rabbit Polyclonal (IgG) to Human CHEK2 / CHK2
Rabbit, Mouse, Dog, Bovine, Rat, Pig, Horse, Human, Monkey
IHC, WB
Unconjugated
50µg  $435.00

CHEK2 / CHK2 Antibody LS-C331943
Rabbit Polyclonal (IgG) to Human CHEK2 / CHK2
Mouse, Rat, Human
ICC, IF, IHC, IP, WB
Unconjugated
50µl  $235.00; 100µl  $315.00; 200µl  $425.00
Immunohistochemistry of paraffin-embedded human lung cancer tissue.
Immunofluorescence analysis of MCF7 cells.

IHC-plus™ CHEK2 / CHK2 Antibody (clone 73C175.1.1) LS-B1321
Mouse Monoclonal [clone 73C175.1.1] (IgG1) to Human CHEK2 / CHK2
Human
IHC, IHC-P, WB
Unconjugated
50µg  $425.00
Anti-CHEK2 / CHK2 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
Western blot of Chk2 in 293 cell lysate. A protein band of approximate molecular weight of 60-62 kDa is detected with antibody at 2 ug/ml.

IHC-plus™ CHEK2 / CHK2 Antibody (clone 3E10) LS-B6213
Mouse Monoclonal [clone 3E10] (IgG2a,k) to Human CHEK2 / CHK2
Human
ELISA, IHC, IHC-P, WB
Unconjugated
50µg  $545.00
Anti-CHEK2 / CHK2 antibody IHC of human colon. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 1.5 ug/ml.
Western blot of CHEK2 expression in HeLa NE.

CHEK2 / CHK2 Antibody (phospho-Thr68) LS-C416418
Rabbit Polyclonal to Human CHEK2 / CHK2
Mouse, Rat, Human
IHC, WB
Unconjugated
100µg  $305.00
Western blot of Chk2 phosphorylation expression in UV treated Jurkat whole cell lysates,The lane on the left is treated with the antigen-specific peptide.

CHEK2 / CHK2 Antibody (clone OTI10G7) LS-C114496
Mouse Monoclonal [clone OTI10G7] (IgG1) to Human CHEK2 / CHK2
Dog, Human, Monkey
Flo, IF, IHC, IHC-P, WB
Unconjugated
100µl 0.66mg/ml  $345.00
IHC of paraffin-embedded liver tissue using anti-CHEK2 mouse monoclonal antibody. (Dilution 1:50).
IHC of paraffin-embedded Ovary tissue using anti-CHEK2 mouse monoclonal antibody. (Dilution 1:50).

CHEK2 / CHK2 Antibody (clone OTI10G7, Carrier-free) LS-C784219
Mouse Monoclonal [clone OTI10G7] (IgG1) to Human CHEK2 / CHK2
Dog, Human, Monkey
Flo, IF, IHC, IP, WB
Unconjugated, Carrier-free
100µg  $395.00

CHEK2 / CHK2 Antibody (clone OTI5C4) LS-C114504
Mouse Monoclonal [clone OTI5C4] (IgG1) to Human CHEK2 / CHK2
Dog, Human
Flo, IF, IHC, IHC-P, IP, WB
Unconjugated
100µl 1.43mg/ml  $345.00
IHC of paraffin-embedded breast tissue using anti-CHEK2 mouse monoclonal antibody. (Dilution 1:50).
IHC of paraffin-embedded colon tissue using anti-CHEK2 mouse monoclonal antibody. (Dilution 1:50).

CHEK2 / CHK2 Antibody (clone OTI5C4, Carrier-free) LS-C784225
Mouse Monoclonal [clone OTI5C4] (IgG1) to Human CHEK2 / CHK2
Dog, Human
Flo, IF, IHC, IP, WB
Unconjugated, Carrier-free
100µg  $395.00

CHEK2 / CHK2 Antibody (clone OTI8D11) LS-C114505
Mouse Monoclonal [clone OTI8D11] (IgG1) to Human CHEK2 / CHK2
Dog, Human, Monkey
Flo, IHC, IHC-P, WB
Unconjugated
100µl 1mg/ml  $345.00
IHC of paraffin-embedded liver tissue using anti-CHEK2 mouse monoclonal antibody. (Dilution 1:50).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CHEK2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CHEK2.

CHEK2 / CHK2 Antibody (clone OTI4C10) LS-C114493
Mouse Monoclonal [clone OTI4C10] (IgG2b) to Human CHEK2 / CHK2
Dog, Human
Flo, IHC, IHC-P, IP, WB
Unconjugated
100µl 1.88mg/ml  $345.00
IHC of paraffin-embedded colon tissue using anti-CHEK2 mouse monoclonal antibody. (Dilution 1:50).
IHC of paraffin-embedded Adenocarcinoma of ovary tissue using anti-CHEK2 mouse monoclonal antibody. (Dilution 1:50).

CHEK2 / CHK2 Antibody (clone OTI2H5) LS-C114492
Mouse Monoclonal [clone OTI2H5] (IgG2b) to Human CHEK2 / CHK2
Dog, Human
Flo, IHC, IHC-P, IP, WB
Unconjugated
100µl 2.18mg/ml  $345.00
IHC of paraffin-embedded colon tissue using anti-CHEK2 mouse monoclonal antibody. (Dilution 1:50).
IHC of paraffin-embedded liver tissue using anti-CHEK2 mouse monoclonal antibody. (Dilution 1:50).

CHEK2 / CHK2 Antibody (clone OTI5C7) LS-C114497
Mouse Monoclonal [clone OTI5C7] (IgG1) to Human CHEK2 / CHK2
Dog, Human
Flo, IHC, IHC-P, IP, WB
Unconjugated
100µl 0.98mg/ml  $345.00
IHC of paraffin-embedded colon tissue using anti-CHEK2 mouse monoclonal antibody. (Dilution 1:50).
IHC of paraffin-embedded Adenocarcinoma of colon tissue using anti-CHEK2 mouse monoclonal antibody. (Dilution 1:50).

CHEK2 / CHK2 Antibody (clone 1C12B8) LS-C108783
Mouse Monoclonal [clone 1C12B8] (IgG2b) to Human CHEK2 / CHK2
Human
ELISA, IF, IHC, WB
Unconjugated
100µl  $385.00
IHC of paraffin-embedded human lung carcinoma (A), liver carcinoma (B), breast carcinoma (C) and kidney carcinoma (D), showing nuclear localization with DAB staining using CHK2 mouse monoclonal antibody.
Confocal immunofluorescence of HeLa cells using CHK2 mouse monoclonal antibody (green), showing nuclear localization. Red: Actin filaments have been labeled with DY-554 phalloidin.

IHC-plus™ CHEK2 / CHK2 Antibody (aa35-84) LS-B7598
Rabbit Polyclonal (IgG) to Human CHEK2 / CHK2
Mouse, Rat, Human
IHC, IHC-P, Peptide-ELISA, WB
Unconjugated
50µl 1mg/ml  $425.00
Anti-CHEK2 / CHK2 antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:100.
Western blot analysis of lysates from Jurkat and HeLa cells treated with etoposide 25uM 24hours, using Chk2 Antibody. The lane on the right is blocked with the synthesized peptide.

CHEK2 / CHK2 Antibody (N-Terminus) LS-C353337
Rabbit Polyclonal to Human CHEK2 / CHK2
Pig, Human
ICC, IF, IHC, IHC-P, WB
Unconjugated
100µl 1mg/ml  $245.00
Immunohistochemical analysis of CHK2 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
Immunofluorescent analysis of CHK2 staining in HL60 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

CHEK2 / CHK2 Antibody LS-C746740
Rabbit Polyclonal (IgG) to Human CHEK2 / CHK2
Mouse, Human
IF, IHC, IP, WB
Unconjugated
50µl  $235.00; 100µl  $295.00; 200µl  $385.00
Immunohistochemistry of paraffin-embedded human lung cancer using CHEK2 antibody at dilution of 1:100 (40x lens).
Immunofluorescence analysis of HeLa cells using CHEK2 antibody. Blue: DAPI for nuclear staining.

CHEK2 / CHK2 Antibody (phospho-Thr68) LS-C14450
Rabbit Polyclonal (IgG) to Human CHEK2 / CHK2
Human, Monkey
ELISA, Flo, ICC, IF, IP, WB
Unconjugated
100µl  $575.00

CHEK2 / CHK2 Antibody (phospho-Ser33/Ser35) LS-C14464
Rabbit Polyclonal (IgG) to Human CHEK2 / CHK2
Human
WB
Unconjugated
100µl  $575.00

CHEK2 / CHK2 Antibody LS-C496663
Rabbit Polyclonal (IgG) to Human CHEK2 / CHK2
Human
Flo, IF, IHC, WB
Unconjugated
100µl  $295.00; 200µl  $385.00
Western blot analysis of extracts of various cell lines, using CHEK2 antibody at 1:1000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. An ECL Kit was used for detection and the exposure time was 30s.

CHEK2 / CHK2 Antibody (clone DCS-270) LS-C343638
Mouse Monoclonal [clone DCS-270] (IgG2a) to Human CHEK2 / CHK2
Human
IHC, IHC-P, IP, WB
Unconjugated
100µg  $345.00
Immunohistochemistry of paraffin-embedded human intestine stained with Chk2 Monoclonal Antibody (DCS-270).
Immunohistochemistry of paraffin-embedded human intestine stained with Chk2 Monoclonal Antibody (DCS-270).

CHEK2 / CHK2 Antibody (phospho-Thr387) LS-C800894
Rabbit Polyclonal (IgG) to Human CHEK2 / CHK2
Mouse, Rat, Human
ELISA, IF, IHC, WB
Unconjugated
100µl 1mg/ml  $325.00; 200µl 1mg/ml  $375.00
Showing 1-25 of 165 results

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For RESEARCH USE ONLY. Not intended for human diagnostic or therapeutic purposes.

The data on this page has been compiled from LifeSpan internal sources, the National Center for Biotechnology Information (NCBI), and The Universal Protein Resource (UniProt).