HCG Beta antibody LS-C392192 is an unconjugated mouse monoclonal antibody to human hCG Beta (CGB). Validated for IHC.
(tested or 100% immunogen sequence identity)
Also available Unmodified
CGB / hCG Beta antibody was raised against recombinant hCG beta protein.
The applications listed have been tested for the unmodified form of this product. Other forms have not been tested. HCG is a glycoprotein which is secreted in large quantities by normal trophoblasts. It is present only in trace amounts in non-pregnant urine and sera but rises sharply during pregnancy. HCG is composed of two non-identical, non-covalently linked polypeptide chains designated as the alpha and beta subunits. hCG mAb detects cells and tumors of trophoblastic origin such as choriocarcinoma. Large cell carcinoma and adenocarcinoma of the lung demonstrate anti-hCG positivity in 90% and 60% of cases respectively. 20% of lung squamous cell carcinomas are positive. hCG expression by non-trophoblastic tumors may indicate aggressive behavior. The optimal dilution of the HCG-beta antibody for each application should be determined by the researcher. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
PBS, 0.01% BSA, 0.05% sodium azide