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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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CDC25B Antibody (aa377‑566, Biotin) LS‑C301213
CDC25B antibody LS-C301213 is a biotin-conjugated rabbit polyclonal antibody to human CDC25B. Validated for IHC and WB.
Catalog
Size
Price
LS-C301213-100
100 µl (0.5 mg/ml)
$455
Description
CDC25B antibody LS-C301213 is a biotin-conjugated rabbit polyclonal antibody to human CDC25B. Validated for IHC and WB.
Target
Human CDC25B
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Biotin. Also available Unconjugated or conjugated with FITC, FITC, Cy3, HRP, APC, PE, APC, Cy7.
Purification
Antigen-specific affinity chromatography followed by Protein A affinity chromatography
Modifications
Unmodified
Applications
  • IHC
  • Western blot (1:100 - 1:400)
  • (applications tested for the base form of this product only)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
CDC25B antibody was raised against recombinant CDC25B (Glu377-Gln566) expressed in E. coli.
Epitope
aa377-566
Specificity
The antibody is a rabbit polyclonal antibody raised against CDC25B. It has been selected for its ability to recognize CDC25B in immunohistochemical staining and western blotting.
Usage
The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
Presentation
PBS, pH 7.4, 0.02% Sodium Azide, 50% Glycerol
Storage
Avoid repeated freeze/thaw cycles. Store at 4°C for frequent use. Aliquot and store at -20°C for 12 months.
Restrictions
For research use only.
About CDC25B
P30305 NM_021872 NP_068658.1

Popular CDC25B Products

Antibody
Species: Human
Applications: IHC, Western blot, ELISA
Anti-CDC25B antibody IHC of human thymus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human, Mouse, Rat, Bovine, Dog, Guinea pig, Horse, Pig
Applications: IHC, IHC - Paraffin, Western blot
Anti-CDC25B antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:50.
Species: Human
Applications: IHC, IHC - Paraffin, Western blot, Immunoprecipitation
CDC25B antibody AVARP03041_P050-NP_068659-CDC25B (cell division cycle 25 homolog B (S. pombe)) Antibody was used in IHC to stain formalin-fixed, paraffin-embedded human liver.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human, Monkey, Dog, Guinea pig, Rabbit
Applications: IHC, IHC - Paraffin, Western blot
Immunofluorescence analysis of HUVEC cells, using CDC25B Antibody. The picture on the right is blocked with the synthesized peptide.
Species: Human, Mouse, Rat
Applications: Immunofluorescence, Western blot, Peptide Enzyme-Linked Immunosorbent Assay

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Images

Western blot

Western blot of recombinant CDC25B.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Western blot of recombinant CDC25B. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Western blot of recombinant CDC25B.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Western blot of recombinant CDC25B. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Western blot of recombinant CDC25B.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Western blot of recombinant CDC25B. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Western blot of recombinant CDC25B.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Western blot of recombinant CDC25B. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Requested From: United States
Date Requested: 11/20/2018
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