CD59 antibody LS-B2359 is an unconjugated mouse monoclonal antibody to human CD59. Validated for Flow, IHC, IP and WB. Tested on 20 paraffin-embedded human tissues.
(tested or 100% immunogen sequence identity)
- IHC - Paraffin (5 µg/ml)
- Western blot (1:10)
- Flow Cytometry
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ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Recognizes human CD59. Reacts with well defined epitope (W40, R-53) on human CD59 molecule.
Immunohistochemistry: LS-B2359 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2359 was determined to be 5 ug/ml. Western Blot: non-reducing. The applications listed have been tested for the unmodified form of this product. Other forms have not been tested.
PBS, 0.1% BSA
Short term: 4°C; Long term: Add glycerol (40-50%) -20°C.
For research use only.