CD59 antibody LS-B5245 is an unconjugated rat monoclonal antibody to human CD59. Validated for Flow, IHC and WB. Tested on 20 paraffin-embedded human tissues.
(tested or 100% immunogen sequence identity)
Also available conjugated with RPE
Protein G purified
- IHC - Paraffin (10 µg/ml)
- IHC - Frozen
- Western blot
- Flow Cytometry (1:50 - 1:100)
- (applications tested for the base form of this product only)
See our complete line of Immunohistochemistry Reagents
including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
CD59 antibody was raised against human peripheral blood T cells.
Recognizes the human CD59 cell surface antigen, an 18-20kD GPI linked glycoprotein broadly expressed by human leucocytes and erythrocytes. Clone YTH53.1 has been shown to block the normal function of CD59. Removal of sodium azide is recommended prior to use in functional assays. XXX recommend the use of EQU003 for this purpose. CD59 is expressed by erythrocytes, and therefore staining in whole blood systems requires a higher concentration of antibody.
Flow Cytometry: Use 10 ul of the suggested working dilution to label 10^6 cells in 100 ul.
PBS, 0.09% Sodium Azide
Store at 4°C or -20°C. Avoid freeze-thaw cycles.
For research use only.