CD53 antibody LS-C121567 is an unconjugated mouse monoclonal antibody to rat CD53. Validated for Flow, IHC and IP.
(tested or 100% immunogen sequence identity)
Also available conjugated with AP
Protein G purified
- IHC - Paraffin
- IHC - Frozen
- Flow Cytometry (1:100 - 1:200)
- (applications tested for the base form of this product only)
See our complete line of Immunohistochemistry Reagents
including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
CD53 antibody was raised against rat T cell blasts.
Recognizes rat CD53 cell surface antigen, a 43kD glycoprotein, which is a member of the transmembrane 4 super family. It is expressed by all myeloid and peripheral lymphoid cells, and by a small subset of thymocytes.
Suitable for use in Flow Cytometry, Immunohistochemistry (frozen), and Immunoprecipitation. Flow Cytometry: 1:100-1:200; 10 ul labels 10^6 cells in 100ul. Immunohistochemistry (paraffin): Requires antigen retrieval using heat treatment prior to staining of paraffin sections.
PBS, pH 7.4, 0.09% Sodium Azide
May be stored at 4°C for short-term only. For long-term storage and to avoid freeze-thaw cycles, aliquot and store at -20°C. Aliquots are stable for up to 1 year at -20°C.
For research use only.