(tested or 100% immunogen sequence identity)
Protein A purified
- IHC - Frozen
- Western blot
- Flow Cytometry
- Functional Assay
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CD53 antibody was raised against leukocytes of patient suffering from a LGL-type leukemia.
Reacts with CD53, a 32-40 kD tetraspanin family glycoprotein exclusively expressed on leukocytes; it is not present on platelets, red blood cells and non-hematopoietic cells. Reacts also with deglycosylated molecule (molecular weight of the antigen is reduced by 15 kD using endoglycosidase F).
Flow Cytometry: Recommended dilution - 4 ug/ml. Immunohistochemistry (frozen sections): It is suitable for discrimination of lymphomas from other tumors. Functional Application: The antibody MEM-53 induces activation of monocytes and B lymphocytes.
PBS, 15 mM sodium azide, approx., pH 7.4.
Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.
For research use only.
This antibody carries the LSBio 100% Guarantee.