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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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CD53 Antibody (clone MEM‑53) LS‑C45824
CD53 antibody LS-C45824 is an unconjugated mouse monoclonal antibody to human CD53. Validated for Flow, Func, IHC, IP and WB.
Antibody
Catalog
Size
Price
LS-C45824-100
100 µg
$255

Popular CD53 Products

Antibody
Species: Human
Applications: Flow Cytometry
Anti-CD53 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4184 concentration 10 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human
Applications: IHC, IHC - Paraffin, IHC - Frozen, Western blot, Immunoprecipitation, Flow Cytometry
Antibody
Species: Human
Applications: IHC - Frozen, Western blot, Immunoprecipitation, Flow Cytometry, Functional Assay
Antibody
Species: Human
Applications: IHC, IHC - Frozen, Immunofluorescence, Flow Cytometry
Antibody
Species: Human
Applications: IHC, IHC - Frozen, Western blot, Immunoprecipitation, Flow Cytometry

Product Description

CD53 antibody LS-C45824 is an unconjugated mouse monoclonal antibody to human CD53. Validated for Flow, Func, IHC, IP and WB.
About CD53
CD53 is a member of the transmembrane 4 superfamily, also known as the tetraspanin family. Most of these members are cell-surface proteins that are characterized by the presence of four hydrophobic domains. The proteins mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility. This encoded protein is a cell surface glycoprotein that is known to complex with integrins. P19397 NM_000560 NP_000551.1

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Specifications

Target
Human CD53
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG1 Monoclonal [MEM-53]
Conjugations
Unconjugated
Purification
Purified by protein-A affinity chromatography. Greater than 95% by SDS-PAGE.
Modifications
Unmodified
Applications
  • IHC
  • IHC - Frozen
  • Western blot
  • Immunoprecipitation
  • Flow Cytometry
  • Functional Assay
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
CD53 antibody was raised against leukocytes of pacient suffering from a LGL-type leukemia.
Specificity
The antibody MEM-53 reacts with CD53, a 32-40 kDa tetraspanin family glycoprotein exclusively expressed on leukocytes; it is not present on platelets, red blood cells and non-hematopoietic cells. The antibody MEM-53 reacts also with deglycosylated molecule (molecular weight of the antigen is reduced by 15 kDa using endoglycosidase F).
Usage
Flow Cytometry: Recommended dilution - 4 ug/ml. Immunohistochemistry (frozen sections): It is suitable for discrimination of lymphomas from other tumors. Functional Application: The antibody MEM-53 induces activation of monocytes and B lymphocytes.
Presentation
PBS, pH 7.4, 15 mM Sodium Azide
Storage
Store at 2°C to 8°C. Do not freeze.
Restrictions
For research use only.

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Requested From: United States
Date Requested: 9/18/2018

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