(tested or 100% immunogen sequence identity)
Protein G purified
Also available Azide-free
- Immunofluorescence (0.5 - 1 µg/ml)
- Flow Cytometry (0.5 - 1 µg/10E6 cells)
- (applications tested for the base form of this product only)
CD53 antibody was raised against stimulated human leukocytes were used as the immunogen for the CD53 antibody.
Recognizes a protein of 33-55kDa, identified as CD53 (Workshop VI. Code N-L033). It is expressed on monocytes and macrophages, dendritic cells, osteoblasts and osteoclasts, and on T and B cells from every stage of differentiation but is absent from platelets, red blood cells. CD53 appears to be the marker with widest reactivity as well as the marker with the strictest specificity to hematopoietic cells. CD53 is a type III membrane with both termini in the cytoplasm and two loops in the extracellular environment. This molecule, in common with other members of tetraspan family, is involved in cellular activation as part of a signal transduction complex involving other membrane glycoproteins. CD53 crosslinking induces calcium flux on human monocyte and B cells. Cross-linking of CD53 promotes activation of resting human B-lymphocytes. This mAb recognizes CD53 transfected cells and partially inhibits T-cell proliferation induced by CD3 antibody (clone: UCHT1). Optimal dilution of the CD53 antibody should be determined by the researcher.
0.01% BSA, 0.05% sodium azide
Store at 2°C to 8°C.
For research use only.
This antibody carries the LSBio 100% Guarantee.