CD53 antibody LS-B4184 is an unconjugated mouse monoclonal antibody to human CD53. Validated for Flow, IHC, IP and WB. Tested on 20 paraffin-embedded human tissues.
(tested or 100% immunogen sequence identity)
Also available conjugated with FITC
Protein G purified
- IHC - Paraffin (10 µg/ml)
- IHC - Frozen
- Western blot
- Flow Cytometry (1:10 - 1:25)
- (applications tested for the base form of this product only)
See our complete line of Immunohistochemistry Reagents
including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunohistochemistry: LS-B4184 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B4184 was determined to be 10 ug/ml. Flow Cytometry: Use 10 ul of the suggested working dilution to label 10^6 cells in 100 ul.
PBS, pH 7.4, 0.09% Sodium Azide
Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.
For research use only.