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Catalog Number Size Price
LS-C746730-20 20 µl $275 
LS-C746730-100 100 µl (2.68 mg/ml) $389 
CD4 Antibody - Immunohistochemistry of paraffin-embedded human lung cancer using CD4 antibody at dilution of 1:150 (40x lens).
CD4 Antibody - Immunohistochemistry of paraffin-embedded mouse spleen using CD4 antibody at dilution of 1:150 (40x lens).
CD4 Antibody - Immunohistochemistry of paraffin-embedded mouse thymus using CD4 antibody at dilution of 1:100 (40x lens).
CD4 Antibody - Immunohistochemistry of paraffin-embedded rat spleen using CD4 antibody at dilution of 1:100 (40x lens).
CD4 Antibody - Immunofluorescence analysis of PC12 cells using CD4 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
CD4 Antibody - Immunofluorescence analysis of NIH/3T3 cells using CD4 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
CD4 Antibody - Immunofluorescence analysis of HeLa cells using CD4 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
CD4 Antibody - Western blot analysis of extracts of various cell lines, using CD4 antibody at 1:1000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. An ECL Kit was used for detection and the exposure time was 90s.
CD4 Antibody - Immunohistochemistry of paraffin-embedded human lung cancer using CD4 antibody at dilution of 1:150 (40x lens).
CD4 Antibody - Immunohistochemistry of paraffin-embedded mouse spleen using CD4 antibody at dilution of 1:150 (40x lens).
CD4 Antibody - Immunohistochemistry of paraffin-embedded mouse thymus using CD4 antibody at dilution of 1:100 (40x lens).
CD4 Antibody - Immunohistochemistry of paraffin-embedded rat spleen using CD4 antibody at dilution of 1:100 (40x lens).
CD4 Antibody - Immunofluorescence analysis of PC12 cells using CD4 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
CD4 Antibody - Immunofluorescence analysis of NIH/3T3 cells using CD4 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
CD4 Antibody - Immunofluorescence analysis of HeLa cells using CD4 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
CD4 Antibody - Western blot analysis of extracts of various cell lines, using CD4 antibody at 1:1000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. An ECL Kit was used for detection and the exposure time was 90s.
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Polyclonal Rabbit anti‑Human CD4 Antibody (IHC, IF, WB) LS‑C746730

Polyclonal Rabbit anti‑Human CD4 Antibody (IHC, IF, WB) LS‑C746730

Antibody:
CD4 Rabbit anti-Human Polyclonal Antibody
Application:
IHC, IF, WB
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified
Price
Catalog Number
$275
LS-C746730-20
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
CD4 Rabbit anti-Human Polyclonal Antibody
Application:
IHC, IF, WB
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified

Specifications

Description
CD4 antibody LS-C746730 is an unconjugated rabbit polyclonal antibody to CD4 from human. It is reactive with human, mouse and rat. Validated for IF, IHC and WB.
Target
Human CD4
Synonyms
CD4 | CD4 antigen | CD4 receptor | CD4mut | CD4 antigen (p55) | CD4 molecule
Host
Rabbit
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Affinity purified
Modifications
Unmodified
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 26-200 of human CD4 (NP_000607.1). KKVVLGKKGDTVELTCTASQKKSIQFHWKNSNQIKILGNQGSFLTKGPSKLNDRADSRRSLWDQGNFPLIIKNLKIEDSDTYICEVEDQKEEVQLLVFGLTANSDTHLLQGQSLTLTLESPPGSSPSVQCRSPRGKNIQGGKTLSVSQLELQDSGTWTCTVLQNQKKVEFKIDIV
Applications
  • IHC (1:50 - 1:200)
  • Immunofluorescence (1:50 - 1:200)
  • Western blot (1:500 - 1:2000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
The predicted MW is 51kDa, while the observed MW by Western blot was 55kDa.
Presentation
PBS, pH 7.3, 0.02% Sodium Azide, 50% Glycerol
Storage
Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About CD4
P01730 NM_000616 NP_000607.1

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 4/29/2024