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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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CD274 / B7‑H1 / PD‑L1 Antibody (clone MIH2, FITC) LS‑C188353
PD-L1 antibody LS-C188353 is an FITC-conjugated mouse monoclonal antibody to human PD-L1 (CD274 / B7-H1). Validated for Flow. Cited in 1 publication.
Catalog
Size
Price
LS-C188353-100
100 µg (0.1 mg/ml)
$355
Description
PD-L1 antibody LS-C188353 is an FITC-conjugated mouse monoclonal antibody to human PD-L1 (CD274 / B7-H1). Validated for Flow. Cited in 1 publication.
Target
Human CD274 / B7-H1 / PD-L1
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG1 Monoclonal [MIH2]
Conjugations
FITC
Purification
Protein G purified
Modifications
Unmodified
Applications
  • Flow Cytometry (1:1 - 1:10)
Immunogen
Human CD274 transfected P815 cells.
Specificity
Detects human CD274, also known as B7-H1 and PD-1L, a cell surface glycoprotein which is a member of the B7 family of co-stimulatory molecules. CD274 is expressed constitutively on macrophages and dendritic cells, and is induced on activated T-cells, B-cells, endothelial cells and epithelial cells in response to Interferons alpha, beta and gamma. CD274 is reported to possess dual functions; inhibition of activated effector T cells and co-stimulation of naive T cells. CD274 inhibits proliferation of activated T cells via ligation to the co-inhibitory molecule CD279 (programmed death-1; PD-1) leading to the secretion of the regulatory cytokine interleukin-10. CD274 has also been shown to costimulate early T cell priming and differentiation. Deregulated CD274 function has been reported in chronic viral and intracellular bacterial infection, as well as in many autoimmune diseases and cancers.
Usage
The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
Presentation
PBS, 0.09% Sodium Azide, 1% BSA
Storage
Short term: store at 4°C. Long term: -20°C. Avoid freeze-thaw cycles. Protect from light.
Restrictions
For research use only.
About CD274 / B7-H1 / PD-L1
Q9NZQ7 NM_014143 NP_054862.1

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Flow cytometry of B7-H1 / PD-L1 / CD274 antibody This image was taken for the unconjugated form of this product. Other forms have not been tested.
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Publications (1)

1
EML4-ALK enhances programmed cell death-ligand 1 expression in pulmonary adenocarcinoma via hypoxia-inducible factor (HIF)-1a and STAT3. Koh J, Jang JY, Keam B, Kim S, Kim MY, Go H, Kim TM, Kim DW, Kim CW, Jeon YK, Chung DH. Oncoimmunology. 2016 5:e1108514. (Flo; Human) [Full Text Article] [PubMed:27141364]

Customer Reviews (0)


Images

Flow Cytometry

Flow cytometry of human peripheral blood monocytes with Mouse anti-Human CD274:FITC This image was taken for the unconjugated form of this product. Other forms have not been tested.
Flow cytometry of human peripheral blood monocytes with Mouse anti-Human CD274:FITC This image was taken for the unconjugated form of this product. Other forms have not been tested.

Flow Cytometry

Flow cytometry of human peripheral blood monocytes with Mouse anti-Human CD274:FITC This image was taken for the unconjugated form of this product. Other forms have not been tested.
Flow cytometry of human peripheral blood monocytes with Mouse anti-Human CD274:FITC This image was taken for the unconjugated form of this product. Other forms have not been tested.

Flow Cytometry

Flow cytometry of human peripheral blood monocytes with Mouse anti-Human CD274:FITC This image was taken for the unconjugated form of this product. Other forms have not been tested.
Flow cytometry of human peripheral blood monocytes with Mouse anti-Human CD274:FITC This image was taken for the unconjugated form of this product. Other forms have not been tested.

Flow Cytometry

Flow cytometry of human peripheral blood monocytes with Mouse anti-Human CD274:FITC This image was taken for the unconjugated form of this product. Other forms have not been tested.
Flow cytometry of human peripheral blood monocytes with Mouse anti-Human CD274:FITC This image was taken for the unconjugated form of this product. Other forms have not been tested.

Requested From: United States
Date Requested: 12/9/2018
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