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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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CD274 / B7‑H1 / PD‑L1 Antibody (clone ANC6H1, RPE) LS‑C357637
PD-L1 antibody LS-C357637 is an RPE-conjugated mouse monoclonal antibody to human PD-L1 (CD274 / B7-H1). Validated for ELISA and Flow.
Catalog
Size
Price
LS-C357637-120
120 tst
$465
Description
PD-L1 antibody LS-C357637 is an RPE-conjugated mouse monoclonal antibody to human PD-L1 (CD274 / B7-H1). Validated for ELISA and Flow.
Target
Human CD274 / B7-H1 / PD-L1
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG1,k Monoclonal [ANC6H1]
Conjugations
R. Phycoerythrin. Also available Unconjugated or conjugated with Biotin, FITC.
Purification
Protein A purified
Modifications
Unmodified
Applications
  • Flow Cytometry
  • ELISA
  • (applications tested for the base form of this product only)
Immunogen
Recombinant human CD274(B7-H1).
Specificity
Recombinant CD274 and native cell surface CD274 expressed on stimulated PBMC.
Usage
Ficoll prepared human peripheral blood mononuclear cells (PBMC) were stimulated overnight in culture with 5 ug/ml PHA. Five x 105 cells per tube were washed and pre incubated 5 minutes with 20^l of 250^g/ml human IgG (to block non specific binding), after which they were incubated 45 minutes on ice with 80 ^l of anti-human CD274 at a dilution factor of 1:50 (20 ^g/ml). They were washed three times, fixed and analyzed by FACS. Cells stained positive with a mean shift of 0.67 log10 fluorescent units when compared to a Mouse IgG1/R-PE negative control at a similar concentration. Binding was blocked when cells were pre incubated 10 minutes with 20 ^l of 0.5 mg/ml anti-CD274 antibody. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
Presentation
50 mM Sodium Phosphate, pH 7.5, 150 mM NaCl, 500 mM KCl, 0.04% Sodium Azide, 15% Glycerol, 0.2% BSA
Storage
Store at 4°C. Do not freeze. Protect from light.
Restrictions
For research use only.
About CD274 / B7-H1 / PD-L1
Q9NZQ7 NM_014143 NP_054862.1

Popular CD274 / B7-H1 / PD-L1 Products

Flow Cytometry Image
Species: Human
Applications: IHC, IHC - Paraffin, Flow Cytometry
Flow cytometry of B7-H1 / PD-L1 / CD274 antibody This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human
Applications: Flow Cytometry, ELISA
Flow cytometry of B7-H1 / PD-L1 / CD274 antibody This image was taken for the unmodified form of this product. Other forms have not been tested.
Species: Human
Applications: Flow Cytometry, ELISA
Flow cytometry of B7-H1 / PD-L1 / CD274 antibody
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Anti-B7-H1 / PD-L1 / CD274 antibody IHC staining of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:100.
Species: Human
Applications: IHC, IHC - Paraffin, Western blot

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Images

Flow Cytometry

Flow cytometry of B7-H1 / PD-L1 / CD274 antibody This image was taken for the unconjugated form of this product. Other forms have not been tested.
Flow cytometry of B7-H1 / PD-L1 / CD274 antibody This image was taken for the unconjugated form of this product. Other forms have not been tested.

Flow Cytometry

Flow cytometry of B7-H1 / PD-L1 / CD274 antibody This image was taken for the unconjugated form of this product. Other forms have not been tested.
Flow cytometry of B7-H1 / PD-L1 / CD274 antibody This image was taken for the unconjugated form of this product. Other forms have not been tested.

Flow Cytometry

Flow cytometry of B7-H1 / PD-L1 / CD274 antibody This image was taken for the unconjugated form of this product. Other forms have not been tested.
Flow cytometry of B7-H1 / PD-L1 / CD274 antibody This image was taken for the unconjugated form of this product. Other forms have not been tested.

Flow Cytometry

Flow cytometry of B7-H1 / PD-L1 / CD274 antibody This image was taken for the unconjugated form of this product. Other forms have not been tested.
Flow cytometry of B7-H1 / PD-L1 / CD274 antibody This image was taken for the unconjugated form of this product. Other forms have not been tested.

Requested From: United States
Date Requested: 12/11/2018
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