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Catalog Number Size Price
LS-C357637-120 120 Tests $501 
CD274 / B7-H1 / PD-L1 Antibody - Flow cytometry of B7-H1 / PD-L1 / CD274 antibody This image was taken for the unconjugated form of this product. Other forms have not been tested.

Monoclonal Mouse anti‑Human CD274 / B7‑H1 / PD‑L1 Antibody (clone ANC6H1, RPE) LS‑C357637

Monoclonal Mouse anti‑Human CD274 / B7‑H1 / PD‑L1 Antibody (clone ANC6H1, RPE) LS‑C357637

Antibody:
CD274 / B7-H1 / PD-L1 Mouse anti-Human Monoclonal (RPE) (ANC6H1) Antibody
Application:
Flo, ELISA
Reactivity:
Human
Format:
R. Phycoerythrin, Unmodified
Other formats:
Price
Catalog Number
$501
LS-C357637-120
Toll Free North America
866-819-4732
For Research Use Only

Overview

Antibody:
CD274 / B7-H1 / PD-L1 Mouse anti-Human Monoclonal (RPE) (ANC6H1) Antibody
Application:
Flo, ELISA
Reactivity:
Human
Format:
R. Phycoerythrin, Unmodified
Other formats:

Specifications

Description
PD-L1 antibody LS-C357637 is an RPE-conjugated mouse monoclonal antibody to human PD-L1 (CD274 / B7-H1). Validated for ELISA and Flow.
Target
Human CD274 / B7-H1 / PD-L1
Synonyms
CD274 | B7-H | B7 homolog 1 | B7-H1 | B7H1 | CD274 molecule | CD274 antigen | PDCD1 ligand 1 | PDL1 | PD-L1 | PDCD1L1 | PDCD1LG1 | Programmed death ligand 1
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG1,k Monoclonal
Clone
ANC6H1
Conjugations
R. Phycoerythrin. Also available Unconjugated or conjugated with Biotin, FITC.
Purification
Protein A purified
Modifications
Unmodified
Immunogen
Recombinant human CD274(B7-H1).
Specificity
Recombinant CD274 and native cell surface CD274 expressed on stimulated PBMC.
Applications
  • Flow Cytometry
  • ELISA
  • Applications tested for the base form of this product only
Usage
Ficoll prepared human peripheral blood mononuclear cells (PBMC) were stimulated overnight in culture with 5 ug/ml PHA. Five x 105 cells per tube were washed and pre incubated 5 minutes with 20^l of 250^g/ml human IgG (to block non specific binding), after which they were incubated 45 minutes on ice with 80 ^l of anti-human CD274 at a dilution factor of 1:50 (20 ^g/ml). They were washed three times, fixed and analyzed by FACS. Cells stained positive with a mean shift of 0.67 log10 fluorescent units when compared to a Mouse IgG1/R-PE negative control at a similar concentration. Binding was blocked when cells were pre incubated 10 minutes with 20 ^l of 0.5 mg/ml anti-CD274 antibody. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
Presentation
50 mM Sodium Phosphate, pH 7.5, 150 mM NaCl, 500 mM KCl, 0.04% Sodium Azide, 15% Glycerol, 0.2% BSA
Storage
Store at 4°C. Do not freeze. Protect from light.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About CD274 / B7-H1 / PD-L1
Q9NZQ7 NM_014143 NP_054862.1

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 12/1/2021