PD-L1 antibody LS-C357681 is an FITC-conjugated mouse monoclonal antibody to human PD-L1 (CD274 / B7-H1). Validated for ELISA and Flow.
Human CD274 / B7-H1 / PD-L1
(tested or 100% immunogen sequence identity)
Also available Unconjugated
or conjugated with Biotin
Protein A purified
- Flow Cytometry
- (applications tested for the base form of this product only)
Recombinant human CD274(B7-H1).
Recombinant CD274 and native cell surface CD274 expressed on stimulated PBMC.
Ficoll prepared human peripheral blood mononuclear cells (PBMC) were stimulated overnight in culture with 5 ug/ml PHA. Five x 105 cells per tube were washed and pre incubated 5 minutes with 20^l of 250^g/ml human IgG (to block non specific binding), after which they were incubated 45 minutes on ice with 80 ^l of anti-human CD274/FITC at a 1:50 dilution (10 ^g/ml). They were washed three times, fixed and analyzed by FACS. Cells stained positive with a mean shift of 0.5 log10 fluorescent units when compared to a Mouse IgG1/FITC negative control at a similar concentration. Binding was blocked when cells were pre incubated 10 minutes with 20 ^l of 0.5 mg/ml anti-CD274 antibody. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
50 mM Sodium Phosphate, pH 7.5, 150 mM NaCl, 100 mM KCl, 0.04% Sodium Azide, 5% Glycerol, 0.2% BSA
Store at 4°C. Do not freeze. Protect from light.
For research use only.
About CD274 / B7-H1 / PD-L1