CARS antibody LS-C101832 is an unconjugated rabbit polyclonal antibody to CARS (CARS1) from human, mouse, rat and other species. Validated for WB.
Human CARS1 / CARS
Human, Monkey, Mouse, Rat, Pig, Rabbit, Zebrafish
(tested or 100% immunogen sequence identity)
Also available conjugated with Biotin
- Western blot (0.2 - 1 µg/ml)
- (applications tested for the base form of this product only)
CARS1 / CARS antibody was raised against synthetic peptide located between aa756-805 of human CARS (Q5HYE4, NP_001014437). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Monkey, Rat (100%); Galago, Dog, Bovine, Horse, Guinea pig, Chicken, Lizard (92%); Turkey (91%); Slime mold (90%); Mouse, Drosophila (85%); Salmon, Zebrafish (84%); Xenopus, Stickleback (83%).
- Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C101832. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Western Blot: Suggested dilution at 1µg/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:1562500.
PBS, 0.09% sodium azide, 2% sucrose
Short term: Store at 2-8°C. Long term: Aliquot and store at -20°C. Avoid freeze/thaw cycles.
For research use only.