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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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CARS1 / CARS Antibody (aa756‑805, HRP) LS‑C440044
CARS antibody LS-C440044 is an HRP-conjugated rabbit polyclonal antibody to CARS (CARS1) from human, mouse, rat and other species. Validated for WB.
Catalog
Size
Price
LS-C440044-100
100 µl (0.65 mg/ml)
$385

Popular CARS1 / CARS Products

Human Brain, Cortex: Formalin-Fixed, Paraffin-Embedded (FFPE)
Species: Human, Mouse
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot
Immunohistochemical analysis of CARS staining in mouse heart formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
Species: Human
Applications: IHC, IHC - Paraffin, ICC, Immunofluorescence, Western blot
Antibody
Species: Human
Applications: IHC, Immunofluorescence, Western blot, ELISA
Immunohistochemistry of paraffin-embedded mouse lung using CARS Antibody at dilution of 1:100 (40x lens).
Species: Human, Mouse, Rat
Applications: IHC, Immunofluorescence, Western blot
CARS antibody LS-C30358 was used in IHC to stain formalin-fixed, paraffin-embedded human lung.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human, Monkey, Bovine, Pig, Rabbit
Applications: IHC, IHC - Paraffin, Western blot

Product Description

CARS1 / CARS Antibody (aa756-805, HRP) for WB/Western LS-C440044

Specifications

Target
Human CARS1 / CARS
Synonyms
CARS, Cysteinyl-tRNA synthetase, CYSRS, Cysteine translase, CARS1, MGC:11246
Host
Rabbit
Reactivity
Human, Chimpanzee, Monkey, Mouse, Rat, Pig, Rabbit, Zebrafish (tested or 100% immunogen sequence identity)
Predicted
Bovine, Dog, Guinea pig, Horse, Chicken (at least 90% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Horseradish Peroxidase. Also available Unconjugated or conjugated with Biotin, FITC.
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • Western blot
  • (applications tested for the base form of this product only)
Immunogen
CARS1 / CARS antibody was raised against synthetic peptide located between aa756-805 of human CARS (Q5HYE4, NP_001014437). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Monkey, Rat (100%); Galago, Dog, Bovine, Horse, Guinea pig, Chicken, Lizard (92%); Turkey (91%); Slime mold (90%); Mouse, Drosophila (85%); Salmon, Zebrafish (84%); Xenopus, Stickleback (83%).
Epitope
aa756-805
Specificity
Human CARS
Usage
The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
Presentation
100 mM sodium phosphate, pH 7.6, 150 mM NaCl
Storage
Short term: store at 4°C. Long term: add glycerol to 40-50%, aliquot to avoid freeze-thaw cycles, and store at -20°C. Protect from light.
Restrictions
For research use only.
About CARS1 / CARS
CARS1 / CARS encodes a class 1 aminoacyl-tRNA synthetase, cysteinyl-tRNA synthetase. Each of the twenty aminoacyl-tRNA synthetases catalyzes the aminoacylation of a specific tRNA or tRNA isoaccepting family with the cognate amino acid. This gene is one of several located near the imprinted gene domain on chromosome 11p15.5, an important tumor-suppressor gene region. P49589 NM_001751 NP_001742.1


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Images

Western blot

CARS antibody LS-C101832 Western blot of MCF7 cell lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
CARS antibody LS-C101832 Western blot of MCF7 cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

CARS antibody LS-C101832 Western blot of MCF7 cell lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
CARS antibody LS-C101832 Western blot of MCF7 cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

CARS antibody LS-C101832 Western blot of MCF7 cell lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
CARS antibody LS-C101832 Western blot of MCF7 cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

CARS antibody LS-C101832 Western blot of MCF7 cell lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
CARS antibody LS-C101832 Western blot of MCF7 cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Requested From: United States
Date Requested: 10/16/2018

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