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Catalog Number Size Price
LS-C287260-10 10 µl $295 
LS-C287260-100 100 µl (1 mg/ml) $520 
BUB3 Antibody - Detection of Human BUB3 by Immunohistochemistry. Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-BUB3 used at a dilution of 1:1000 (1 ug/ml).
BUB3 Antibody - Detection of human Bub3 by western blot. Samples: Whole cell lysate (50 µg) from HeLa cells treated with Nocodazole (+) or mock treated (-). Antibody: Affinity purified rabbit anti-Bub3 antibody used for WB at 0.066 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
BUB3 Antibody - Detection of Human BUB3 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa cells treated with Nocodazole (+) or mock treated (-). Antibody: Affinity purified rabbit anti-BUB3 antibody used at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 minutes.
BUB3 Antibody - Detection of Human BUB3 by Immunohistochemistry. Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-BUB3 used at a dilution of 1:1000 (1 ug/ml).
BUB3 Antibody - Detection of human Bub3 by western blot. Samples: Whole cell lysate (50 µg) from HeLa cells treated with Nocodazole (+) or mock treated (-). Antibody: Affinity purified rabbit anti-Bub3 antibody used for WB at 0.066 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
BUB3 Antibody - Detection of Human BUB3 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa cells treated with Nocodazole (+) or mock treated (-). Antibody: Affinity purified rabbit anti-BUB3 antibody used at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 minutes.
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Polyclonal Rabbit anti‑Human BUB3 Antibody (aa275‑325, IHC, WB) LS‑C287260

Polyclonal Rabbit anti‑Human BUB3 Antibody (aa275‑325, IHC, WB) LS‑C287260

Antibody:
BUB3 Rabbit anti-Human Polyclonal (aa275-325) Antibody
Application:
IHC, WB
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$295
LS-C287260-10
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
BUB3 Rabbit anti-Human Polyclonal (aa275-325) Antibody
Application:
IHC, WB
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
BUB3 antibody LS-C287260 is an unconjugated rabbit polyclonal antibody to human BUB3 (aa275-325). Validated for IHC and WB.
Target
Human BUB3
Synonyms
BUB3 | Budding uninhibited by benomyl | HBUB3 | BUB3L | Mitotic checkpoint component
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Predicted
Orangutan, Mouse, Bovine (at least 90% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Affinity purified
Modifications
Unmodified
Immunogen
A portion of GeneID: 9184 corresponding to amino acids between 275 and 325 of UniprotID: O43684
Epitope
aa275-325
Specificity
Region between residues 275 and 325 of human Bub3 budding uninhibited by benzimidazoles 3 using the numbering given in Swiss-Prot entry O43684 (GeneID 9184).
Applications
  • IHC (1:500 - 1:2000)
  • Western blot (1:5000 - 1:15000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
IHC: Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
Manufacturer
Bethyl Laboratories, Inc.
Presentation
Tris-citrate/phosphate buffer, pH 7-8, 0.09% sodium azide.
Storage
Store at 2-8°C for up to one year.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About BUB3
O43684 NM_004725 NP_004716.1

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 4/26/2024