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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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BRAF / B-Raf Antibody (clone 1H12) IHC-plus™ LS-B3862

Note: This antibody replaces LS-C108748, LS-C758759, LS-C355318
Anti-BRAF antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:200.
Anti-BRAF antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:200.
Anti-BRAF antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:200.
Confocal immunofluorescence of MCF-7 (left) and HepG2 (right) cells using BRAF mouse monoclonal antibody (green). Red: Actin filaments have been labeled with DY-554 phalloidin. Blue: DRAQ5 fluorescent DNA dye
Western blot using BRAF mouse monoclonal antibody against HeLa (1), HL60 (2), HepG2 (3) and NIH/3T3 (4) cell lysate.
Anti-BRAF antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:200.
Anti-BRAF antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:200.
Anti-BRAF antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:200.
Confocal immunofluorescence of MCF-7 (left) and HepG2 (right) cells using BRAF mouse monoclonal antibody (green). Red: Actin filaments have been labeled with DY-554 phalloidin. Blue: DRAQ5 fluorescent DNA dye
Western blot using BRAF mouse monoclonal antibody against HeLa (1), HL60 (2), HepG2 (3) and NIH/3T3 (4) cell lysate.
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BRAF / B-Raf Antibody (clone 1H12) IHC-plus™ LS-B3862

Note: This antibody replaces LS-C108748, LS-C758759, LS-C355318
Antibody:
Mouse Monoclonal to Human BRAF / B-Raf
Application:
IHC, IHC-P, IF, WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$425 
LS-B3862-50
Toll Free North America
866-819-4732

Overview

Antibody:
Mouse Monoclonal to Human BRAF / B-Raf
Application:
IHC, IHC-P, IF, WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
B-Raf antibody LS-B3862 is an unconjugated mouse monoclonal antibody to human B-Raf (BRAF). Validated for ELISA, IF, IHC and WB. Tested on 20 paraffin-embedded human tissues.

Target
Human BRAF / B-Raf
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG1 Monoclonal
Clone
1H12
Conjugations
Unconjugated
Purification
Ascites
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (1:200 - 1:300)
  • Immunofluorescence (1:200 - 1:1000)
  • Western blot (1:100 - 1:500)
  • ELISA (1:10000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
BRAF / B-Raf antibody was raised against purified recombinant fragment of human BRAF expressed in E. Coli encoding aa 285-401.
Specificity
Human, Mouse
Usage
Immunohistochemistry: LS-B3861 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working dilution for LS-B3861 was determined to be 1:200.
Presentation
Ascitic fluid containing 0.03% sodium azide
Storage
Short term: store at 4°C. Long term: store at -20°C.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About BRAF / B-Raf
P15056 NM_004333 NP_004324.2

Validation

Anti-BRAF antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:200.
IHC Validated
Anti-BRAF antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:200.
Anti-BRAF antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:200.
Anti-BRAF antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:200.
IHC Validated
Anti-BRAF antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:200.
Anti-BRAF antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:200.
Anti-BRAF antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:200.
IHC Validated
Anti-BRAF antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:200.
Anti-BRAF antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:200.

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Requested From: United States
Date Requested: 6/18/2019
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