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Immunohistochemistry Services

Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

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Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Catalog Number Size Price
LS-C104439-500 500 µg $255 
Antibody

Monoclonal BMP4 Antibody (WB) LS‑C104439

Monoclonal BMP4 Antibody (WB) LS‑C104439

Note: This antibody replaces LS-C84808, LS-C44861
Antibody:
Mouse Monoclonal to Human BMP4
Application:
WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$255 
LS-C104439-500
500 µg
Toll Free North America
866-819-4732
For Research Use Only

Overview

Antibody:
Mouse Monoclonal to Human BMP4
Application:
WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
BMP4 antibody LS-C104439 is an unconjugated mouse monoclonal antibody to human BMP4. Validated for ELISA and WB.
Target
Human BMP4
Synonyms
BMP4 | BMP-2B | BMP-4 | BMP2B | BMP2B1 | Bone morphogenetic protein 2B | Bone morphogenetic protein 4 | DVR4 | MCOPS6 | OFC11 | Zyme
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG2b Monoclonal
Conjugations
Unconjugated
Purification
Protein G purified
Modifications
Unmodified
Applications
  • Western blot (0.25 - 0.5 µg/ml)
  • ELISA (4 - 8 µg/ml)
Immunogen
E.coli derived recombinant mature Human BMP-4.
Specificity
Human BMP-4
Usage
Immunohistochemistry: This antibody stained HepG2 cells. The primary antibody was incubated at 3 ug/ml overnight at 4°C followed by a fluorescent labeled secondary antibody. Optimal concentrations and conditions may vary. Information courtesy of the Cell Profiling group, SciLifeLab Stockholm. Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of Human BMP-4 (5 ng/ml), a concentration of 0.4-0.6 ug/ml of this antibody is required. ELISA: In a sandwich ELISA (assuming 100 ul/well), a concentration of 4-8 ug/ml of this antibody will detect at least 2000 pg/ml of recombinant human BMP-4 when used in conjunction with compatible secondary reagents. Western Blot: To detect hBMP-4 by Western Blot analysis this antibody can be used at a concentration of 0.25-0.50 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hBMP-4 is 2-4 ng/lane, under non-reducing conditions.
Presentation
Lyophilized from PBS
Reconstitution
Reconstitute in sterile water to 1 mg/ml.
Storage
Store Lyophilized at room temperature up to 1 month; Reconstituted for up to 2 weeks at 2°C to 8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About BMP4
P12644 NM_001202 NP_001193.2

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Requested From: United States
Date Requested: 4/4/2020