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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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BBC3 / PUMA Antibody LS‑C779

Catalog Number Size Price
LS-C779-100 100 µg (1 mg/ml) $335 
PUMA expression in human dysplastic nevi (premalignant melanoma).
Western blot analysis for Puma a(CT) using antibody at 1:500 against 30 ug/lane of A)Jurkat and B) NIH 3T3whole cell lysate.
PUMA expression in human dysplastic nevi (premalignant melanoma).
Western blot analysis for Puma a(CT) using antibody at 1:500 against 30 ug/lane of A)Jurkat and B) NIH 3T3whole cell lysate.
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BBC3 / PUMA Antibody LS‑C779

Antibody:
Rabbit Polyclonal to Human BBC3 / PUMA
Application:
IHC, IHC-P, WB
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$335 
LS-C779-100
Toll Free North America
866-819-4732

Overview

Antibody:
Rabbit Polyclonal to Human BBC3 / PUMA
Application:
IHC, IHC-P, WB
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
PUMA antibody LS-C779 is an unconjugated rabbit polyclonal antibody to human PUMA (BBC3 ). Validated for IHC and WB.
Target
Human BBC3 / PUMA
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Predicted
Monkey (at least 90% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Protein G purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin
  • Western blot (1:500 - 1:1000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
A portion of amino acids 186-199 (PLPRGHRAPEMEPN) of human PUMA a. Percent identity by BLAST analysis: Human, Monkey (100%); Marmoset (93%); Ferret, Panda, Dog, Horse, Pig (86%); Mouse, Rat (79%).
Specificity
The amino acid sequence used as immunogen is 100% homologous in human (PUMA a and b) and 78% homologous in mouse and rat. Human PUMA a is a 193 amino acid protein with a predicted molecular weight of approximately 22 kD. Human PUMA b is a 131 amino acid protein with a predicted molecular weight of approximately 15 kD.
Presentation
PBS, 0.05% Sodium Azide, 0.2% Gelatin
Storage
Short term: 4°C; Long term: -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About BBC3 / PUMA
Q9BXH1 NM_014417 NP_055232.1

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Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with Puma BH3 Domain antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Species: Human, Mouse
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot, Flow Cytometry
Reactivity: Human
Range: 0.156-10 ng/ml
Reactivity: Human, Mouse, Rat
Range: 0.1-1000 ng/ml

Requested From: United States
Date Requested: 8/23/2019
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