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Immunohistochemistry Services

Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

TCR Screening Services

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Cell Cycle Pathways
Protein Family And Group
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Catalog Number Size Price
LS-C779-100 100 µg (1 mg/ml) $335 
BBC3 / PUMA Antibody - PUMA expression in human dysplastic nevi (premalignant melanoma).
BBC3 / PUMA Antibody - Western blot analysis for Puma a(CT) using antibody at 1:500 against 30 ug/lane of A)Jurkat and B) NIH 3T3whole cell lysate.
BBC3 / PUMA Antibody - PUMA expression in human dysplastic nevi (premalignant melanoma).
BBC3 / PUMA Antibody - Western blot analysis for Puma a(CT) using antibody at 1:500 against 30 ug/lane of A)Jurkat and B) NIH 3T3whole cell lysate.
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Polyclonal BBC3 / PUMA Antibody (IHC, WB) LS‑C779

Polyclonal BBC3 / PUMA Antibody (IHC, WB) LS‑C779

Antibody:
Rabbit Polyclonal to Human BBC3 / PUMA
Application:
IHC, IHC-P, WB
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$335 
LS-C779-100
100 µg
1 mg/ml
Toll Free North America
866-819-4732

Overview

Antibody:
Rabbit Polyclonal to Human BBC3 / PUMA
Application:
IHC, IHC-P, WB
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
PUMA antibody LS-C779 is an unconjugated rabbit polyclonal antibody to human PUMA (BBC3 ). Validated for IHC and WB.
Target
Human BBC3 / PUMA
Synonyms
BBC3 | Bcl-2-binding component 3 | BCL2 binding component 3 | JFY-1 | JFY1 | PUMA | PUMA alpha
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Predicted
Monkey (at least 90% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Protein G purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin
  • Western blot (1:500 - 1:1000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
A portion of amino acids 186-199 (PLPRGHRAPEMEPN) of human PUMA a. Percent identity by BLAST analysis: Human, Monkey (100%); Marmoset (93%); Ferret, Panda, Dog, Horse, Pig (86%); Mouse, Rat (79%).
Specificity
The amino acid sequence used as immunogen is 100% homologous in human (PUMA a and b) and 78% homologous in mouse and rat. Human PUMA a is a 193 amino acid protein with a predicted molecular weight of approximately 22 kD. Human PUMA b is a 131 amino acid protein with a predicted molecular weight of approximately 15 kD.
Presentation
PBS, 0.05% Sodium Azide, 0.2% Gelatin
Storage
Short term: 4°C; Long term: -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About BBC3 / PUMA
Q9BXH1 NM_014417 NP_055232.1

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Requested From: United States
Date Requested: 2/25/2020