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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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BBC3 / PUMA

BCL2 binding component 3

Apoptosis is related to many diseases and development. The p53 tumor-suppressor protein induces apoptosis through transcriptional activation of several genes. A novel p53 inducible pro-apoptotic gene was identified recently and designated PUMA (for p53 upregulated modulator of apoptosis) and bbc3 (for Bcl-2 binding component 3) in human and mouse. PUMA/bbc3 is one of the pro-apoptotic Bcl-2 family members including Bax and Noxa, which are also transcriptional targets of p53. The PUMA gene encodes two BH3 domain-containing proteins termed PUMA-a and PUMA-b. PUMA proteins bind Bcl-2, localize to the mitochondria, and induce cytochrome c release and apoptosis in response to p53. PUMA may be a direct mediator of p53-induced apoptosis.

Gene Name: BCL2 binding component 3
Family/Subfamily: Apoptosis , not assigned-Apoptosis
Synonyms: BBC3, Bcl-2-binding component 3, BCL2 binding component 3, JFY-1, JFY1, PUMA, PUMA alpha
Target Sequences: NM_014417 NP_055232.1 Q9BXH1

Publications (1)

1
Aberrant activation of notch signaling in human breast cancer. Stylianou S, Clarke RB, Brennan K. Cancer research. 2006 66:1517-25. [PubMed:16452208]

Filtered By: None
Target
BBC3 / PUMA (120)
Reactivity
Human (104)
Mouse (52)
Rat (46)
Gibbon (3)
Monkey (1)
Pig (1)
Application
IHC (52)
IHC-P (8)
WB (119)
Flo (2)
ELISA (20)
ICC (18)
IF (7)
IP (3)
Peptide-ELISA (3)
Host
rabbit (115)
mouse (4)
goat (1)
Isotype
IgG (57)
Clonality
monoclonal mc (5)
polyclonal pc (115)
Clone
10C5G1 (1)
10D4G7 (1)
2A8F6 (1)
2A9G5 (1)
Format
AP Conjugated (6)
APC (1)
APC Conjugated (6)
Atto 390 Conjugated (2)
Atto 488 Conjugated (2)
Atto 565 Conjugated (2)
Atto 594 Conjugated (4)
Atto 633 Conjugated (2)
Atto 655 Conjugated (2)
Atto 680 Conjugated (2)
Atto 700 Conjugated (2)
Biotin Conjugated (11)
Cy3 Conjugated (1)
Cy7 Conjugated (1)
DY488 Conjugated (1)
DY550 Conjugated (1)
DY650 Conjugated (1)
FITC Conjugated (10)
HRP Conjugated (6)
PE (2)
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PerCP Conjugated (2)
RPE Conjugated (2)
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Unconjugated (46)
Epitope
C-Terminus (30)
N-Terminus (19)
aa109-137 (8)
aa119-154 (8)
aa21-70 (4)
aa76-170 (4)
Arg169 (1)
C-terminal (1)
Internal (1)
aa120-169 (1)
aa145-159 (1)
aa150-193 (1)
aa158-192 (1)
aa178-187 (1)
aa2-16 (1)
Publications
No (120)
Showing 1-25 of 120 results

BBC3 / PUMA Antibody (C-Terminus) IHC-plus™ LS-B1334
Rabbit Polyclonal (IgG) to Human BBC3 / PUMA
Mouse, Human
ICC IF IHC IHC-P WB
Unconjugated
50µg 1mg/ml  $425.00
Anti-BBC3 / PUMA antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
Anti-BBC3 / PUMA antibody IHC of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
Anti-BBC3 / PUMA antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
Anti-BBC3 / PUMA antibody IHC of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.

BBC3 / PUMA Antibody (aa119-154) LS-C98860
Rabbit Polyclonal to Human BBC3 / PUMA
Mouse, Human
Flo IF IHC IHC-P WB
Unconjugated
400µl  $305.00
Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with Puma BH3 Domain antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Confocal immunofluorescence of Puma BH3 Domain Antibody with HeLa cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with Puma BH3 Domain antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Confocal immunofluorescence of Puma BH3 Domain Antibody with HeLa cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

BBC3 / PUMA Antibody LS-C497865
Rabbit Monoclonal to Human BBC3 / PUMA
Mouse, Rat, Human
Flo IF IHC WB
Unconjugated
50µl  $295.00; 100µl  $385.00; 200µl  $560.00
Immunohistochemistry of paraffin-embedded mouse stomach using BBC3 antibodyat dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human mammary cancer using BBC3 antibodyat dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse stomach using BBC3 antibodyat dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human mammary cancer using BBC3 antibodyat dilution of 1:100 (40x lens).

BBC3 / PUMA Antibody LS-C779
Rabbit Polyclonal (IgG) to Human BBC3 / PUMA
Human
IHC IHC-P WB
Unconjugated
100µg 200µl 0.5mg/ml  $335.00
PUMA expression in human dysplastic nevi (premalignant melanoma).
Western blot analysis for Puma a(CT) using antibody at 1:500 against 30 ug/lane of A)Jurkat and B) NIH 3T3whole cell lysate.
PUMA expression in human dysplastic nevi (premalignant melanoma).
Western blot analysis for Puma a(CT) using antibody at 1:500 against 30 ug/lane of A)Jurkat and B) NIH 3T3whole cell lysate.

BBC3 / PUMA Antibody (aa2-16) IHC-plus™ LS-B7285
Rabbit Polyclonal (IgG) to Human BBC3 / PUMA
Mouse, Rat, Human
IHC IHC-P WB
Unconjugated
50µg 1mg/ml  $425.00
Anti-BBC3 / PUMA antibody IHC of human colon. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 2.5 ug/ml.
Anti-BBC3 / PUMA antibody IHC of human colon. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 2.5 ug/ml.

BBC3 / PUMA Antibody IHC-plus™ LS-B8733
Rabbit Polyclonal (IgG) to Human BBC3 / PUMA
Mouse, Rat, Human
IHC IHC-P WB
Unconjugated
50µg 0.5mg/ml  $425.00
Anti-PUMA antibody IHC staining of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-PUMA antibody IHC staining of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

BBC3 / PUMA Antibody (C-Terminus) LS-C9
Rabbit Polyclonal (IgG) to Human BBC3 / PUMA
Human
IF IHC WB
Unconjugated
100µg 1mg/ml  $365.00
Immunohistochemistry of PUMA in human breast carcinoma with PUMA antibody at 10 ug/ml.
Immunohistochemistry of PUMA in human breast tissue with PUMA antibody at 2.5 µg/ml.
Immunohistochemistry of PUMA in human breast carcinoma with PUMA antibody at 10 ug/ml.
Immunohistochemistry of PUMA in human breast tissue with PUMA antibody at 2.5 µg/ml.

BBC3 / PUMA Antibody IHC-plus™ LS-B270
Rabbit Polyclonal to Human BBC3 / PUMA
Human
IHC IHC-P
Unconjugated
50µg 1mg/ml  $425.00
Anti-BBC3 / PUMA antibody IHC of human colon. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-BBC3 / PUMA antibody IHC of human colon. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

BBC3 / PUMA Antibody LS-C393454
Rabbit Polyclonal to Human BBC3 / PUMA
Mouse, Rat, Human
IF IHC IHC-P WB
Unconjugated
100µl  $305.00

BBC3 / PUMA Antibody (aa109-137) LS-C319790
Rabbit Polyclonal (IgG) to Human BBC3 / PUMA
Human
ELISA IHC WB
Unconjugated
200µl  $530.00

BBC3 / PUMA Antibody (aa109-137, HRP) LS-C540366
Rabbit Polyclonal (IgG) to Human BBC3 / PUMA
Human
ELISA IHC WB
HRP Conjugated
200µl  $680.00

BBC3 / PUMA Antibody (aa109-137, FITC) LS-C212937
Rabbit Polyclonal (IgG) to Human BBC3 / PUMA
Human
ELISA IHC WB
FITC Conjugated
200µl  $680.00

BBC3 / PUMA Antibody (aa109-137, PE) LS-C212936
Rabbit Polyclonal (IgG) to Human BBC3 / PUMA
Human
ELISA IHC WB
PE Conjugated
200µl  $680.00

BBC3 / PUMA Antibody (aa109-137, AP) LS-C212933
Rabbit Polyclonal (IgG) to Human BBC3 / PUMA
Human
ELISA IHC WB
AP Conjugated
200µl  $680.00

BBC3 / PUMA Antibody (aa109-137, APC) LS-C212934
Rabbit Polyclonal (IgG) to Human BBC3 / PUMA
Human
ELISA IHC WB
APC Conjugated
200µl  $680.00

BBC3 / PUMA Antibody (aa119-154) LS-C328416
Rabbit Polyclonal (IgG) to Human BBC3 / PUMA
Mouse, Human
ELISA IHC WB
Unconjugated
200µl 0.25mg/ml  $530.00

BBC3 / PUMA Antibody (aa119-154, HRP) LS-C549107
Rabbit Polyclonal (IgG) to Human BBC3 / PUMA
Mouse, Human
ELISA IHC WB
HRP Conjugated
200µl  $680.00

BBC3 / PUMA Antibody (aa119-154, Biotin) LS-C529014
Rabbit Polyclonal (IgG) to Human BBC3 / PUMA
Mouse, Human
ELISA IHC WB
Biotin Conjugated
200µl  $680.00

BBC3 / PUMA Antibody (aa119-154, FITC) LS-C220486
Rabbit Polyclonal (IgG) to Human BBC3 / PUMA
Mouse, Human
ELISA IHC WB
FITC Conjugated
200µl  $680.00

BBC3 / PUMA Antibody (aa119-154, PE) LS-C220485
Rabbit Polyclonal (IgG) to Human BBC3 / PUMA
Mouse, Human
ELISA IHC WB
PE Conjugated
200µl  $680.00

BBC3 / PUMA Antibody (aa119-154, AP) LS-C221202
Rabbit Polyclonal (IgG) to Human BBC3 / PUMA
Mouse, Human
ELISA IHC WB
AP Conjugated
200µl  $680.00

BBC3 / PUMA Antibody (aa119-154, APC) LS-C220483
Rabbit Polyclonal (IgG) to Human BBC3 / PUMA
Mouse, Human
ELISA IHC WB
APC Conjugated
200µl  $680.00

BBC3 / PUMA Antibody LS-C402160
Rabbit Polyclonal (IgG) to Human BBC3 / PUMA
Human
ELISA IHC WB
Unconjugated
20µl  $205.00; 60µl  $235.00; 120µl  $295.00; 200µl  $395.00
Immunohistochemistry of Human colon cancer using BBC3 Polyclonal Antibody at dilution of 1:20.
Western blot analysis of HeLa cell, using BBC3 Polyclonal Antibody at dilution of 1:300.
Immunohistochemistry of Human colon cancer using BBC3 Polyclonal Antibody at dilution of 1:20.
Western blot analysis of HeLa cell, using BBC3 Polyclonal Antibody at dilution of 1:300.

BBC3 / PUMA Antibody LS-C332261
Rabbit Polyclonal (IgG) to Human BBC3 / PUMA
Mouse, Rat, Human
IHC IP WB
Unconjugated
50µl  $235.00; 100µl  $295.00; 200µl  $385.00
Western blot analysis of extracts of various cell lines.
Western blot analysis of extracts of various cell lines.

BBC3 / PUMA Antibody (aa109-137) LS-C156751
Rabbit Polyclonal to Human BBC3 / PUMA
Human
IHC IHC-P WB
Unconjugated
400µl  $305.00
BBC3 Antibody immunohistochemistry of formalin-fixed and paraffin-embedded human cervix tissue followed by peroxidase-conjugated secondary antibody and DAB staining.
BBC3 Antibody western blot of A549 cell line lysates (35 ug/lane). The BBC3 antibody detected the BBC3 protein (arrow).
BBC3 Antibody immunohistochemistry of formalin-fixed and paraffin-embedded human cervix tissue followed by peroxidase-conjugated secondary antibody and DAB staining.
BBC3 Antibody western blot of A549 cell line lysates (35 ug/lane). The BBC3 antibody detected the BBC3 protein (arrow).
Showing 1-25 of 120 results

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For RESEARCH USE ONLY. Not intended for human diagnostic or therapeutic purposes.

The data on this page has been compiled from LifeSpan internal sources, the National Center for Biotechnology Information (NCBI), and The Universal Protein Resource (UniProt).

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