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BAIAP2 / IRSP53 Antibody LS‑C482424

Catalog Number Size Price
LS-C482424-50 50 µl (1 mg/ml) $255 
LS-C482424-100 100 µl (1 mg/ml) $295 
LS-C482424-200 200 µl (1 mg/ml) $375 
Immunohistochemical analysis of IRSp53 staining in rat kidney formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
Immunofluorescent analysis of IRSp53 staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 deg C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
Western blot analysis of IRSp53 expression in A549 (A); K562 (B); SW620 (C) whole cell lysates.
Immunohistochemical analysis of IRSp53 staining in rat kidney formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
Immunofluorescent analysis of IRSp53 staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 deg C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
Western blot analysis of IRSp53 expression in A549 (A); K562 (B); SW620 (C) whole cell lysates.
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BAIAP2 / IRSP53 Antibody LS‑C482424

Antibody:
Rabbit Polyclonal to Human BAIAP2 / IRSP53
Application:
IHC, IHC-P, ICC, IF, WB
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified
Price
Catalog Number
$255 
LS-C482424-50
Toll Free North America
866-819-4732

Overview

Antibody:
Rabbit Polyclonal to Human BAIAP2 / IRSP53
Application:
IHC, IHC-P, ICC, IF, WB
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified

Specifications

Description
IRSP53 antibody LS-C482424 is an unconjugated rabbit polyclonal antibody to IRSP53 (BAIAP2 ) from human, mouse and rat. Validated for ICC, IF, IHC and WB.
Target
Human BAIAP2 / IRSP53
Host
Rabbit
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (1:50 - 1:200)
  • ICC (1:50 - 1:200)
  • Immunofluorescence (1:50 - 1:200)
  • Western blot (1:500 - 1:2000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
Recombinant full length human IRSp53.
Specificity
Recognizes endogenous levels of IRSp53 protein.
Presentation
PBS, pH 7.3, 0.01% Sodium Azide, 30% Glycerol
Storage
Aliquot and store at -20°C for 1 year. Avoid freeze/thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About BAIAP2 / IRSP53
Q9UQB8 NM_017450 NP_059344.1

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Requested From: United States
Date Requested: 9/19/2019