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Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Catalog Number Size Price
LS-B8289-50 50 µg (0.5 mg/ml) $425 
BAIAP2 / IRSP53 Antibody - Anti-BAIAP2 / IRSP53 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 3.75 ug/ml.
BAIAP2 / IRSP53 Antibody - HEK293 lysate (10ug protein in RIPA buffer) over expressing Human BAIAP2 with DYKDDDDK tag probed with (1.0 ug/ml) in Lane A and probed with anti- DYKDDDDK Tag (1/3000) in lane C. Mock-transfected HEK293 probed (1mg/ml) in Lane B. Pri
BAIAP2 / IRSP53 Antibody - BAIAP2 antibody staining (1 ug/ml) of Human Brain (Cerebellum) lysate (RIPA buffer, 35g total protein per lane). Primary incubated for 12 hour. Detected by Western blot of chemiluminescence.
BAIAP2 / IRSP53 Antibody - Anti-BAIAP2 / IRSP53 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 3.75 ug/ml.
BAIAP2 / IRSP53 Antibody - HEK293 lysate (10ug protein in RIPA buffer) over expressing Human BAIAP2 with DYKDDDDK tag probed with (1.0 ug/ml) in Lane A and probed with anti- DYKDDDDK Tag (1/3000) in lane C. Mock-transfected HEK293 probed (1mg/ml) in Lane B. Pri
BAIAP2 / IRSP53 Antibody - BAIAP2 antibody staining (1 ug/ml) of Human Brain (Cerebellum) lysate (RIPA buffer, 35g total protein per lane). Primary incubated for 12 hour. Detected by Western blot of chemiluminescence.
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IHCPlus™ Polyclonal BAIAP2 / IRSP53 Antibody (Isoform 3, IHC, WB) LS‑B8289

IHCPlus™ Polyclonal BAIAP2 / IRSP53 Antibody (Isoform 3, IHC, WB) LS‑B8289

Note: This antibody replaces LS-C34628
Antibody:
Goat Polyclonal to Human BAIAP2 / IRSP53
Application:
IHC, IHC-P, WB, Peptide-ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$425 
LS-B8289-50
50 µg
0.5 mg/ml
Toll Free North America
866-819-4732

Overview

Antibody:
Goat Polyclonal to Human BAIAP2 / IRSP53
Application:
IHC, IHC-P, WB, Peptide-ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
IRSP53 antibody LS-B8289 is an unconjugated goat polyclonal antibody to human IRSP53 (BAIAP2 ). Validated for IHC, Peptide-ELISA and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human BAIAP2 / IRSP53
Synonyms
BAIAP2 | BAI-associated protein 2 | BAI1-associated protein 2 | BAP2 | FLAF3 | Fas ligand-associated factor 3 | IRSP53 | IRS-58 | Protein BAP2 | IRSp53/58
Host
Goat
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (3.75 µg/ml)
  • Western blot (1 - 2 µg/ml)
  • Peptide Enzyme-Linked Immunosorbent Assay (1:8000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
Peptide with sequence C-SMSSADVEVARF, from the C-Terminus of protein sequence according to NP_006331.1.
Blocking Peptide
LS-E25244 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-B8289. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
Isoform 3
Specificity
Human BAIAP2 / IRSP53. This antibody will recognise only one of three reported isoforms (NP_006331.1).
Usage
Peptide ELISA: antibody detection limit dilution 1:16000. Western blot: Approx 55 kDa band observed in Human Brain (Cerebellum and Frontal Cortex) lysates (calculated MW of 57.4 kDa according to NP_006331.1). Recommended concentration: 1-3 ug/ml. An additional band of unknown identity was also consistently observed at 150-170 kDa. This band was successfully blocked by incubation with the immunising peptide.
Presentation
TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA
Storage
Aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About BAIAP2 / IRSP53
Q9UQB8 NM_017450 NP_059344.1

Validation

BAIAP2 / IRSP53 Antibody - Anti-BAIAP2 / IRSP53 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 3.75 ug/ml.
Anti-BAIAP2 / IRSP53 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 3.75 ug/ml.
Anti-BAIAP2 / IRSP53 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 3.75 ug/ml.

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Requested From: United States
Date Requested: 2/23/2020