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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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IHCPlus™ BAIAP2 / IRSP53 Antibody Isoform 3 Polyclonal IHC,Peptide‑ELISA,WB LS‑B8289

Catalog Number Size Price
LS-B8289-50 50 µg (0.5 mg/ml) $425 
Anti-BAIAP2 / IRSP53 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 3.75 ug/ml.
HEK293 lysate (10ug protein in RIPA buffer) over expressing Human BAIAP2 with DYKDDDDK tag probed with (1.0 ug/ml) in Lane A and probed with anti- DYKDDDDK Tag (1/3000) in lane C. Mock-transfected HEK293 probed (1mg/ml) in Lane B. Pri
BAIAP2 antibody staining (1 ug/ml) of Human Brain (Cerebellum) lysate (RIPA buffer, 35g total protein per lane). Primary incubated for 12 hour. Detected by Western blot of chemiluminescence.
Anti-BAIAP2 / IRSP53 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 3.75 ug/ml.
HEK293 lysate (10ug protein in RIPA buffer) over expressing Human BAIAP2 with DYKDDDDK tag probed with (1.0 ug/ml) in Lane A and probed with anti- DYKDDDDK Tag (1/3000) in lane C. Mock-transfected HEK293 probed (1mg/ml) in Lane B. Pri
BAIAP2 antibody staining (1 ug/ml) of Human Brain (Cerebellum) lysate (RIPA buffer, 35g total protein per lane). Primary incubated for 12 hour. Detected by Western blot of chemiluminescence.
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IHCPlus™ BAIAP2 / IRSP53 Antibody Isoform 3 Polyclonal IHC,Peptide‑ELISA,WB LS‑B8289

Note: This antibody replaces LS-C34628
Antibody:
Goat Polyclonal to Human BAIAP2 / IRSP53
Application:
IHC, IHC-P, WB, Peptide-ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$425 
LS-B8289-50
Toll Free North America
866-819-4732

Overview

Antibody:
Goat Polyclonal to Human BAIAP2 / IRSP53
Application:
IHC, IHC-P, WB, Peptide-ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
IRSP53 antibody LS-B8289 is an unconjugated goat polyclonal antibody to human IRSP53 (BAIAP2 ). Validated for IHC, Peptide-ELISA and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human BAIAP2 / IRSP53
Host
Goat
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (3.75 µg/ml)
  • Western blot (1 - 2 µg/ml)
  • Peptide Enzyme-Linked Immunosorbent Assay (1:8000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
Peptide with sequence C-SMSSADVEVARF, from the C Terminus of the protein sequence according to NP_006331.1.
Blocking Peptide
LS-E25244 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-B8289. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
Isoform 3
Specificity
Human BAIAP2 / IRSP53. This antibody will recognise only one of three reported isoforms (NP_006331.1).
Usage
Peptide ELISA: antibody detection limit dilution 1:16000. Western blot: Approx 55 kDa band observed in Human Brain (Cerebellum and Frontal Cortex) lysates (calculated MW of 57.4 kDa according to NP_006331.1). Recommended concentration: 1-3 ug/ml. An additional band of unknown identity was also consistently observed at 150-170 kDa. This band was successfully blocked by incubation with the immunising peptide.
Presentation
TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA
Storage
Aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About BAIAP2 / IRSP53
Q9UQB8 NM_017450 NP_059344.1

Validation

Anti-BAIAP2 / IRSP53 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 3.75 ug/ml.
IHC Validated
Anti-BAIAP2 / IRSP53 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 3.75 ug/ml.
Anti-BAIAP2 / IRSP53 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 3.75 ug/ml.

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Anti-BAIAP2 / IRSP53 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Species: Human, Mouse, Rat, Dog, Pig
Applications: IHC, IHC - Paraffin, Western blot, Peptide Enzyme-Linked Immunosorbent Assay
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
Species: Human, Mouse
Applications: IHC, IHC - Paraffin, Western blot
BAIAP2 Antibody western blot of K562 cell line and mouse brain tissue lysates (35 ug/lane). The BAIAP2 antibody detected the BAIAP2 protein (arrow).
Species: Human, Mouse
Applications: Western blot
IHC of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-BAIAP2 mouse monoclonal antibody.
Species: Human
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot, Flow Cytometry
Immunohistochemical analysis of IRSp53 staining in rat kidney formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
Species: Human, Mouse, Rat
Applications: IHC, IHC - Paraffin, ICC, Immunofluorescence, Western blot
Reactivity: Human
Range: 15.6-1000 pg/ml

Requested From: United States
Date Requested: 8/17/2019
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