Human BAIAP2 / IRSP53
(tested or 100% immunogen sequence identity)
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
- IHC - Paraffin (3.75 µg/ml)
- Western blot (1 - 2 µg/ml)
- Peptide Enzyme-Linked Immunosorbent Assay (1:8000)
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Peptide with sequence C-SMSSADVEVARF, from the C Terminus of the protein sequence according to NP_006331.1.
- Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-B8289. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Human BAIAP2 / IRSP53. This antibody will recognise only one of three reported isoforms (NP_006331.1).
Peptide ELISA: antibody detection limit dilution 1:16000. Western blot: Approx 55 kDa band observed in Human Brain (Cerebellum and Frontal Cortex) lysates (calculated MW of 57.4 kDa according to NP_006331.1). Recommended concentration: 1-3 ug/ml. An additional band of unknown identity was also consistently observed at 150-170 kDa. This band was successfully blocked by incubation with the immunising peptide.
TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA
Aliquot and store at -20°C. Avoid freeze-thaw cycles.
For research use only.
This antibody carries the LSBio 100% Guarantee.