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Immunohistochemistry Services

Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

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Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Catalog Number Size Price
LS-B2869-50 50 µg (0.5 mg/ml) $425 
BAIAP2 / IRSP53 Antibody - Anti-BAIAP2 / IRSP53 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
BAIAP2 / IRSP53 Antibody - Antibody staining (1 ug/ml) of Human Brain lysate (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by Western blot of chemiluminescence.
BAIAP2 / IRSP53 Antibody - Anti-BAIAP2 / IRSP53 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
BAIAP2 / IRSP53 Antibody - Antibody staining (1 ug/ml) of Human Brain lysate (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by Western blot of chemiluminescence.
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IHCPlus™ Polyclonal BAIAP2 / IRSP53 Antibody (Isoform 1, IHC, WB) LS‑B2869

IHCPlus™ Polyclonal BAIAP2 / IRSP53 Antibody (Isoform 1, IHC, WB) LS‑B2869

Note: This antibody replaces LS-C54569, LS-C74625
Antibody:
Goat Polyclonal to Human BAIAP2 / IRSP53
Application:
IHC, IHC-P, WB, Peptide-ELISA
Reactivity:
Human, Mouse, Rat, Dog, Pig
Format:
Unconjugated, Unmodified
Price
Catalog Number
$425 
LS-B2869-50
50 µg
0.5 mg/ml
Toll Free North America
866-819-4732
For Research Use Only

Overview

Antibody:
Goat Polyclonal to Human BAIAP2 / IRSP53
Application:
IHC, IHC-P, WB, Peptide-ELISA
Reactivity:
Human, Mouse, Rat, Dog, Pig
Format:
Unconjugated, Unmodified

Specifications

Description
IRSP53 antibody LS-B2869 is an unconjugated goat polyclonal antibody to IRSP53 (BAIAP2) (Isoform 1) from human, mouse, rat and other species. Validated for IHC, Peptide-ELISA and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human BAIAP2 / IRSP53
Synonyms
BAIAP2 | BAI-associated protein 2 | BAI1-associated protein 2 | BAP2 | FLAF3 | Fas ligand-associated factor 3 | IRSP53 | IRS-58 | Protein BAP2 | IRSp53/58
Host
Goat
Reactivity
Human, Mouse, Rat, Dog, Pig (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (5 µg/ml)
  • Western blot (1 - 3 µg/ml)
  • Peptide Enzyme-Linked Immunosorbent Assay (1:64000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
Peptide with sequence C-SGSGTLVSTV, from the C-Terminus of protein sequence according to NP_059344.1.
Blocking Peptide
LS-E25246 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-B2869. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
Isoform 1
Specificity
Human BAIAP2 / IRSP53. This antibody is expected to recognise only human isoform 1 according to NP_059344.1.
Usage
Immunohistochemistry: LS-B2869 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2869 was determined to be 5 ug/ml.
Presentation
TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA
Storage
Aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About BAIAP2 / IRSP53
Q9UQB8 NM_017450 NP_059344.1

Validation

BAIAP2 / IRSP53 Antibody - Anti-BAIAP2 / IRSP53 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-BAIAP2 / IRSP53 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-BAIAP2 / IRSP53 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

LSBio Ratings

IHC-plus™ BAIAP2 / IRSP53 Antibody (Isoform 1) for IHC, WB/Western LS-B2869 has an LSBio Rating of
Laboratory Validation Score (4)

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Requested From: United States
Date Requested: 4/1/2020