BAD antibody LS-C3907 is an unconjugated mouse monoclonal antibody to BAD from human, mouse, rat and other species. Validated for ELISA, IP and WB.
Mouse, Human, Monkey, Rat
(tested or 100% immunogen sequence identity)
- Western blot (1:2000)
- Immunoprecipitation (1:50)
BAD antibody was raised against synthetic phosphopeptide (KLH coupled) corresponding to residues surrounding Ser112 of mouse Bad.
Detects endogenous levels of total Bad only when phosphorylated at Ser112. Does not detect Bad phosphorylated at other sites. Nor does it detect related family members. Species cross-reactivity: Human, mouse, monkey and rat. Other species have not been tested.
Suitable for use in ELISA, Western Blot and Immunoprecipitation. Western Blot: 1:2000, incubate membrane with diluted antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4?C with gentle shaking, overnight. Immunoprecipitation: 1:50.
10 mM HEPES, pH 7.5, 150 mM NaCl, 0.1 mg/ml BSA, 0.02% Sodium Azide, 50% Glycerol
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
For research use only.