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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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BAD

BCL2-associated agonist of cell death

Members in the Bcl-2 family are critical regulators of apoptosis by either inhibiting or promoting cell death. Bcl-2 homology 3 (BH3) domain containing pro-apoptotic proteins, such as Bax, Bid, and Bik, form a growing subclass of the Bcl-2 family. Another such protein is the Bcl-2-antagonist of cell death (Bad). Bad regulates apoptosis by forming heterodimers with anti-apoptotic proteins Bcl-2 and Bcl-xL, thereby preventing them from binding with Bax. Bad activity is regulated by its phosphorylation; it is inactivated by kinases such as Akt and MAP kinase and thus promotes cell survival, whereas JNK-induced phosphorylation promotes the apoptotic role of Bad.

Gene Name: BCL2-associated agonist of cell death
Family/Subfamily: Apoptosis , Not assigned-Other
Synonyms: BAD, BBC2, Bcl-2-like protein 8, BCL2-binding component 6, BCL2L8, BBC6, BCL-X/BCL-2 binding protein, Bcl-2-binding component 6, Bcl2 antagonist of cell death, BCL2-binding protein, Bcl2-L-8
Target Sequences: NM_004322 NP_004313.1 Q92934

Publications (1)

1
Ischemic preconditioning blocks BAD translocation, Bcl-xL cleavage, and large channel activity in mitochondria of postischemic hippocampal neurons. Miyawaki T, Mashiko T, Ofengeim D, Flannery RJ, Noh KM, Fujisawa S, Bonanni L, Bennett MV, Zukin RS, Jonas EA. Proceedings of the National Academy of Sciences of the United States of America. 2008 105:4892-7. (WB, IP) [PubMed:18347331] [PMC:PMC2290755]

Filtered by: None
Target
BAD (342)
Reactivity
Human (262)
Mouse (144)
Rat (79)
Bat (4)
Bovine (9)
Chimpanzee (6)
Dog (1)
Drosophila (1)
Gibbon (6)
Guinea pig (8)
Hamster (1)
Horse (4)
Monkey (15)
Pig (1)
Rabbit (23)
Sheep (1)
Xenopus (4)
Application
IHC (101)
IHC-Fr (3)
IHC-P (28)
WB (165)
Flo (3)
DB (176)
ELISA (226)
ICC (1)
IF (14)
IP (14)
Peptide-ELISA (7)
Host
rabbit (329)
mouse (12)
chicken (1)
Isotype
IgG (253)
IgG1 (10)
IgG2a,l (1)
Clonality
monoclonal mc (14)
polyclonal pc (328)
Clone
1G5B3 (1)
3H8 (1)
Format
AP Conjugated (32)
APC (1)
APC Conjugated (33)
Azide-free (29)
Biotin Conjugated (36)
Cy3 Conjugated (1)
Cy7 Conjugated (1)
FITC Conjugated (35)
HRP Conjugated (35)
PE Conjugated (33)
Preservative Free (2)
Unconjugated (136)
Epitope
pSer134 (17)
pSer75 (17)
pSer99 (17)
pSer112 (11)
pSer118 (10)
pSer155 (9)
pSer91 (9)
aa1-168 (8)
aa53-81 (8)
aa92-127 (8)
pSer109 (8)
pSer111 (8)
pSer124 (8)
pSer161 (8)
pSer25 (8)
pSer32 (8)
pSer34 (8)
pSer57 (8)
pSer71 (8)
pSer74 (8)
pSer96 (8)
pSer97 (8)
pThr137 (8)
pThr80 (8)
pThr94 (8)
pTyr110 (8)
pTyr113 (8)
pTyr76 (8)
Ser118 (7)
C-Terminus (6)
aa65-114 (4)
pSer136 (3)
30-110 aa (2)
70-150 aa (2)
aa1-50 (2)
40-120 aa, Internal (1)
Arg149 (1)
N-Terminus (1)
Phe130 (1)
Pro102 (1)
Pro130 (1)
Ser112 (1)
aa100-149 (1)
aa102-151 (1)
aa115 (1)
aa119-168 (1)
aa2-23 (1)
aa21-70 (1)
aa61-110 (1)
aa87-103 (1)
pSer91/Ser128 (1)
Publications
No (342)
Showing 1-25 of 342 results

BAD Antibody IHC-plus™ LS-B12854
Rabbit Polyclonal to Mouse BAD
Drosophila, Pig, Rat, Hamster, Rabbit, Mouse, Dog, Sheep, Bovine, Monkey
IHC IHC-P WB
Unconjugated
50µg 1mg/ml  $425.00
Human Adrenal: Formalin-Fixed, Paraffin-Embedded (FFPE)

BAD Antibody (phospho-Ser118) LS-C97416
Rabbit Polyclonal to Human BAD
Human
IHC IHC-P WB
Unconjugated
400µl  $325.00
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
The anti-Phospho-Bad-S118 antibody is used in Western blot to detect Phospho-Bad-S118 in HL60 tissue lysate

BAD Antibody (phospho-Ser136) LS-C74589
Rabbit Monoclonal (IgG) to Mouse BAD
Mouse, Human
ELISA WB
Unconjugated
200µl  $590.00

BAD Antibody LS-C497461
Mouse Monoclonal to Human BAD
Mouse, Rat, Human
IF IHC IP WB
Unconjugated
50µl  $265.00; 100µl  $345.00; 200µl  $445.00
Western blot analysis of extracts of HeLa cells, using BAD antibody.

BAD Antibody (phospho-Ser112) LS-C3894
Rabbit Polyclonal (IgG) to Mouse BAD
Mouse, Rat, Human, Monkey
ELISA IP WB
Unconjugated
100µl  $530.00

BAD Antibody IHC-plus™ LS-B461
Rabbit Polyclonal (IgG) to Human BAD
Mouse, Rat, Human
ELISA IF IHC IHC-P WB
Unconjugated
0.05mg 1mg/ml  $425.00
Anti-BAD antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Immunofluorescence of BAD in Rat Thymus cells with BAD antibody at 10 ug/ml.

BAD Antibody (Pro102) LS-C74590
Rabbit Monoclonal (IgG) to Human BAD
Mouse, Rat, Human
ELISA WB
Unconjugated
20µl  $395.00; 100µl  $605.00

BAD Antibody (aa119-168) IHC-plus™ LS-B7566
Rabbit Polyclonal (IgG) to Human BAD
Mouse, Rat, Human
IHC IHC-P Peptide-ELISA WB
Unconjugated
50µl 1mg/ml  $425.00
Anti-BAD antibody IHC of human adrenal. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:100.
Anti-BAD antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:100.

BAD Antibody LS-C358658
Rabbit Polyclonal to Human BAD
Human
ICC IF IHC IP WB
Unconjugated
50µl  $325.00

BAD Antibody (aa61-110) IHC-plus™ LS-B7640
Rabbit Polyclonal (IgG) to Human BAD
Mouse, Rat, Human
IHC IHC-P Peptide-ELISA
Unconjugated
50µl 1mg/ml  $425.00
Anti-BAD antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:100.

BAD Antibody (phospho-Ser134) LS-C198668
Rabbit Polyclonal (IgG) to Human BAD
Mouse, Rat, Human
IF IHC IHC-P Peptide-ELISA WB
Unconjugated
50µl 1mg/ml  $265.00; 100µl 1mg/ml  $325.00
Immunohistochemistry analysis of paraffin-embedded human brain, using BAD (Phospho-Ser134) Antibody. The picture on the right is blocked with the phospho peptide.
Immunofluorescence analysis of HeLa cells, using BAD (Phospho-Ser134) Antibody. The picture on the right is blocked with the phospho peptide.

BAD Antibody (30-110 aa) LS-C381353
Rabbit Polyclonal (IgG) to Human BAD
Mouse, Rat, Human, Monkey
ELISA IF IHC WB
Unconjugated
100µl 1mg/ml  $265.00

BAD Antibody (70-150 aa) LS-C381541
Rabbit Polyclonal (IgG) to Human BAD
Mouse, Rat, Human
ELISA IF IHC WB
Unconjugated
100µl 1mg/ml  $265.00

BAD Antibody (70-150 aa) LS-C382269
Rabbit Polyclonal (IgG) to Human BAD
Mouse, Rat, Human
ELISA IF IHC WB
Unconjugated
100µl 1mg/ml  $265.00

BAD Antibody (phospho-Ser99) LS-C97419
Rabbit Polyclonal to Human BAD
Human
DB IF IHC IHC-P WB
Unconjugated
400µl  $325.00
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
Fluorescent confocal image of HeLa cells stained with phospho-Bad-S99 antibody. HeLa cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated phospho-Bad-S99 primary antibody (1:200, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 5 min). Note the highly specific localization of the phospho-Bad-S99 mainly to the cytoplasm.

BAD Antibody (Ser112) IHC-plus™ LS-B1172
Rabbit Polyclonal (IgG) to Human BAD
Mouse, Rat, Human
IHC IHC-P WB
Unconjugated
50µl 1mg/ml  $425.00
Anti-BAD antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 20 ug/ml.
Western blot of extracts from 293 cells using BAD(Ab-112) antibody and the same antibody preincubated with blocking peptide.

BAD Antibody (aa92-127) LS-C98866
Rabbit Polyclonal to Human BAD
Mouse, Human
Flo IHC IHC-P WB
Unconjugated
400µl  $325.00
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
Western blot of Bad BH3 Domain antibody in mouse bladder tissue lysates (35 ug/lane). Bad (arrow) was detected using the purified antibody.

BAD Antibody (Pro130) LS-C176582
Rabbit Polyclonal to Human BAD
Mouse, Rat, Human
IF IHC IHC-P WB
Unconjugated
100µl  $345.00
IHC of BAD (P130) pAb in paraffin-embedded human brain tissue.
Western blot of BAD (P130) pAb in extracts from NIH/3T3 cells.

BAD Antibody LS-C331583
Rabbit Polyclonal (IgG) to Human BAD
Mouse, Rat, Human
IF IHC WB
Unconjugated
50µl  $235.00; 100µl  $315.00; 200µl  $425.00
Immunohistochemistry of paraffin-embedded rat kidney using BAD antibodyat dilution of 1:200 (40x lens).
Immunofluorescence analysis of HeLa cells using BAD antibody. Blue: DAPI for nuclear staining.

BAD Antibody LS-C148207
Rabbit Polyclonal to Human BAD
Human
Flo IHC IHC-Fr IHC-P IP WB
Unconjugated
50µl  $335.00
Immunofluorescence microscopy of BAD using Polyclonal Antibody to BAD at 1:2000. Du145 human prostate carcinoma cells were cultured without (A) or with (B and C) 1 uM of the triphosphatase inhibitor thapsigargin (THG) for 12 hr. A and B, staining with BAD antibody, followed by a FITC-conjugated secondary antibody. C, staining with a mitochondrial marker (antibody to mitochondrial Hsp60), followed by a rhodamine-conjugated secondary antibody. THG induces Ca2+ release from internal stores which can promote apoptosis. BAD staining was located diffusely throughout the cytoplasm of untreated cells (A), and localized to the mitochondria in treated cells (B).

BAD Antibody LS-C769050
Rabbit Polyclonal (IgG) to Human BAD
Mouse, Rat, Human
ELISA IF IHC-P WB
Unconjugated
20µl 1mg/ml  $195.00; 60µl 1mg/ml  $235.00; 120µl 1mg/ml  $285.00; 200µl 1mg/ml  $375.00

BAD Antibody (aa2-23) LS-C148206
Rabbit Polyclonal to Mouse BAD
Mouse, Rat
IHC IHC-Fr IHC-P IP WB
Unconjugated
50µl  $335.00

BAD Antibody (phospho-Ser99) LS-C326915
Rabbit Polyclonal (IgG) to Human BAD
Human
DB ELISA IHC WB
Unconjugated
200µl  $590.00

BAD Antibody (aa21-70) LS-C121027
Rabbit Polyclonal (IgG) to Mouse BAD
Mouse, Rat, Human
IHC IHC-P Peptide-ELISA WB
Unconjugated
50µl 1mg/ml  $265.00; 100µl 1mg/ml  $325.00
IHC of paraffin-embedded human breast carcinoma tissue, using BAD (Cleaved-Asp71) Antibody. The picture on the right is treated with the synthesized peptide.
Western blot of extracts from 293 cells, treated with Etoposide 25 uM 60', using BAD (Cleaved-Asp71) Antibody. The lane on the right is treated with the synthesized peptide.

BAD Antibody (aa87-103) LS-C148205
Rabbit Polyclonal to Human BAD
Human
IHC IHC-Fr IHC-P IP WB
Unconjugated
50µl  $335.00
Showing 1-25 of 342 results

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PLEASE NOTE

For RESEARCH USE ONLY. Not intended for human diagnostic or therapeutic purposes.

The data on this page has been compiled from LifeSpan internal sources, the National Center for Biotechnology Information (NCBI), and The Universal Protein Resource (UniProt).

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