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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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ATXN3 / JOS Antibody (aa226‑254) LS‑C407637
JOS antibody LS-C407637 is an unconjugated rabbit polyclonal antibody to JOS (ATXN3) from human, rat and dog. Validated for IHC and WB.
Catalog
Size
Price
LS-C407637-100
100 µg
$325

Popular ATXN3 / JOS Products

IHC of paraffin-embedded H661 xenograft using Ataxin 3 antibody at 1:500 dilution.
Species: Human, Mouse
Applications: IHC, IHC - Paraffin, Western blot
Formalin-fixed and paraffin-embedded human brain tissue reacted with ATXN3 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Species: Human
Applications: IHC, IHC - Paraffin, Western blot, Flow Cytometry
Human Heart: Formalin-Fixed, Paraffin-Embedded (FFPE).  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human, Monkey, Mouse, Rat, Bovine, Horse, Pig, Sheep
Applications: IHC, IHC - Paraffin, Western blot
Immunohistochemistry of paraffin-embedded human pancreas tissue using antibody at 1: 100 dilution.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human, Mouse
Applications: IHC, IHC - Paraffin, Western blot, ELISA
Immunofluorescence analysis of HeLa cells using ATXN3 antibody.
Species: Human
Applications: IHC, Immunofluorescence, Western blot

Product Description

JOS antibody LS-C407637 is an unconjugated rabbit polyclonal antibody to JOS (ATXN3) from human, rat and dog. Validated for IHC and WB.
About ATXN3 / JOS
Deubiquitinating enzyme involved in protein homeostasis maintenance, transcription, cytoskeleton regulation, myogenesis and degradation of misfolded chaperone substrates. Binds long polyubiquitin chains and trims them, while it has weak or no activity against chains of 4 or less ubiquitins. P54252 NM_004993 NP_004984.2

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Specifications

Target
Human ATXN3 / JOS
Host
Rabbit
Reactivity
Human, Rat, Dog (tested or 100% immunogen sequence identity)
Predicted
Bovine (at least 90% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunogen affinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (0.5 - 1 µg/ml)
  • Western blot (0.1 - 0.5 µg/ml)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
ATXN3 / JOS antibody was raised against a synthetic peptide corresponding to a sequence at the C-terminus of human Ataxin 3 (226-254aa EEDLQRALALSRQEIDMEDEEADLRRAIQ), different from the related mouse and rat sequences by two amino acids.
Epitope
aa226-254
Specificity
Ubiquitous.
Usage
IHC: Antigen retrieval by boiling the paraffin sections in 10 mM citrate buffer, pH6.0, for 20 minutes is required for the staining of formalin/paraffin sections.
Presentation
Lyophilized from 7.1 mM sodium phosphate, 77 mM NaCl, 2.5% BSA, 0.025% sodium azide
Reconstitution
Reconstitute with 200 µl of distilled water.
Storage
At -20°C for 1 year. After reconstitution, at 4°C for 1 month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid freeze-thaw cycles.
Restrictions
For research use only.

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Images

Immunohistochemistry

Ataxin 3 antibody IHC-paraffin. IHC(P): Human Lung Cancer Tissue.
Ataxin 3 antibody IHC-paraffin. IHC(P): Human Lung Cancer Tissue.

Western blot

Ataxin 3 antibody Western blot. All lanes: Anti Ataxin 3 at 0.5 ug/ml. Lane 1: Rat Brain Tissue Lysate at 50 ug. Lane 2: COLO320 Whole Cell Lysate at 40 ug. Lane 3: HELA Whole Cell Lysate at 40 ug. Predicted band size: 42 kD. Observed band size: 42 kD.
Ataxin 3 antibody Western blot. All lanes: Anti Ataxin 3 at 0.5 ug/ml. Lane 1: Rat Brain Tissue Lysate at 50 ug. Lane 2: COLO320 Whole Cell Lysate at 40 ug. Lane 3: HELA Whole Cell Lysate at 40 ug. Predicted band size: 42 kD. Observed band size: 42 kD.

Immunohistochemistry

Ataxin 3 antibody IHC-paraffin. IHC(P): Human Lung Cancer Tissue.
Ataxin 3 antibody IHC-paraffin. IHC(P): Human Lung Cancer Tissue.

Western blot

Ataxin 3 antibody Western blot. All lanes: Anti Ataxin 3 at 0.5 ug/ml. Lane 1: Rat Brain Tissue Lysate at 50 ug. Lane 2: COLO320 Whole Cell Lysate at 40 ug. Lane 3: HELA Whole Cell Lysate at 40 ug. Predicted band size: 42 kD. Observed band size: 42 kD.
Ataxin 3 antibody Western blot. All lanes: Anti Ataxin 3 at 0.5 ug/ml. Lane 1: Rat Brain Tissue Lysate at 50 ug. Lane 2: COLO320 Whole Cell Lysate at 40 ug. Lane 3: HELA Whole Cell Lysate at 40 ug. Predicted band size: 42 kD. Observed band size: 42 kD.

Immunohistochemistry

Ataxin 3 antibody IHC-paraffin. IHC(P): Human Lung Cancer Tissue.
Ataxin 3 antibody IHC-paraffin. IHC(P): Human Lung Cancer Tissue.

Western blot

Ataxin 3 antibody Western blot. All lanes: Anti Ataxin 3 at 0.5 ug/ml. Lane 1: Rat Brain Tissue Lysate at 50 ug. Lane 2: COLO320 Whole Cell Lysate at 40 ug. Lane 3: HELA Whole Cell Lysate at 40 ug. Predicted band size: 42 kD. Observed band size: 42 kD.
Ataxin 3 antibody Western blot. All lanes: Anti Ataxin 3 at 0.5 ug/ml. Lane 1: Rat Brain Tissue Lysate at 50 ug. Lane 2: COLO320 Whole Cell Lysate at 40 ug. Lane 3: HELA Whole Cell Lysate at 40 ug. Predicted band size: 42 kD. Observed band size: 42 kD.

Immunohistochemistry

Ataxin 3 antibody IHC-paraffin. IHC(P): Human Lung Cancer Tissue.
Ataxin 3 antibody IHC-paraffin. IHC(P): Human Lung Cancer Tissue.

Western blot

Ataxin 3 antibody Western blot. All lanes: Anti Ataxin 3 at 0.5 ug/ml. Lane 1: Rat Brain Tissue Lysate at 50 ug. Lane 2: COLO320 Whole Cell Lysate at 40 ug. Lane 3: HELA Whole Cell Lysate at 40 ug. Predicted band size: 42 kD. Observed band size: 42 kD.
Ataxin 3 antibody Western blot. All lanes: Anti Ataxin 3 at 0.5 ug/ml. Lane 1: Rat Brain Tissue Lysate at 50 ug. Lane 2: COLO320 Whole Cell Lysate at 40 ug. Lane 3: HELA Whole Cell Lysate at 40 ug. Predicted band size: 42 kD. Observed band size: 42 kD.

Requested From: United States
Date Requested: 9/20/2018

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