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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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ATXN3 / JOS Antibody (aa1‑245) LS‑C186388 Best Seller
JOS antibody LS-C186388 is an unconjugated rabbit polyclonal antibody to JOS (ATXN3) from human and mouse. Validated for IHC and WB.
Catalog
Size
Price
LS-C186388-100
100 µl (0.73 mg/ml)
$335

Popular ATXN3 / JOS Products

Formalin-fixed and paraffin-embedded human brain tissue reacted with ATXN3 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Species: Human
Applications: IHC, IHC - Paraffin, Western blot, Flow Cytometry
Human Heart: Formalin-Fixed, Paraffin-Embedded (FFPE).  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human, Monkey, Mouse, Rat, Bovine, Horse, Pig, Sheep
Applications: IHC, IHC - Paraffin, Western blot
Immunohistochemistry of paraffin-embedded human pancreas tissue using antibody at 1: 100 dilution.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human, Mouse
Applications: IHC, IHC - Paraffin, Western blot, ELISA
Immunofluorescence analysis of HeLa cells using ATXN3 antibody.
Species: Human
Applications: IHC, Immunofluorescence, Western blot

Product Description

JOS antibody LS-C186388 is an unconjugated rabbit polyclonal antibody to JOS (ATXN3) from human and mouse. Validated for IHC and WB.
About ATXN3 / JOS
Deubiquitinating enzyme involved in protein homeostasis maintenance, transcription, cytoskeleton regulation, myogenesis and degradation of misfolded chaperone substrates. Binds long polyubiquitin chains and trims them, while it has weak or no activity against chains of 4 or less ubiquitins. P54252 NM_004993 NP_004984.2

ATXN3 Antibody, ATX3 Antibody, Ataxin 3 Antibody, Ataxin 3 variant h Antibody, AT3 Antibody, Ataxin 3 variant m Antibody, Ataxin 3 variant ref Antibody, Ataxin-3 Antibody, Olivopontocerebellar ataxia 3 Antibody, SCA3 Antibody, MJD1 Antibody, JOS Antibody, Josephin Antibody, MJD Antibody


Specifications

Target
Human ATXN3 / JOS
Host
Rabbit
Reactivity
Human, Mouse (tested or 100% immunogen sequence identity)
Predicted
Rat, Bovine, Pig (at least 90% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (1:100 - 1:1000)
  • Western blot (1:500 - 1:3000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
ATXN3 / JOS antibody was raised against recombinant fragment corresponding to a region within amino acids 1 and 245 of Ataxin 3 (SwissProt P54252). Percent identity by BLAST analysis: Human (100%); Pig (99%); Bovine (98%); Rat (96%).
Epitope
aa1-245
Specificity
Human ATXN3 / JOS
Usage
IHC-paraffin: Suggested antigen retrieval using heat mediated 10 mM Citrate buffer (pH 6.0) or Tris-EDTA buffer (pH 8.0).
Presentation
0.1 M Tris-glycine, pH 7.0, 20% glycerol, 0.01% Thimerosal
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Restrictions
For research use only.

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Customer Reviews (0)


Images

Immunohistochemistry

IHC of paraffin-embedded H661 xenograft using Ataxin 3 antibody at 1:500 dilution.
IHC of paraffin-embedded H661 xenograft using Ataxin 3 antibody at 1:500 dilution.

Western blot

Sample (50 ug of whole cell lysate). A: mouse brain. 10% SDS PAGE. ATXN3 / JOS antibody diluted at 1:500.
Sample (50 ug of whole cell lysate). A: mouse brain. 10% SDS PAGE. ATXN3 / JOS antibody diluted at 1:500.

Western blot

Sample (30 ug of whole cell lysate). A: U87-MG. 10% SDS PAGE. ATXN3 / JOS antibody diluted at 1:1000.
Sample (30 ug of whole cell lysate). A: U87-MG. 10% SDS PAGE. ATXN3 / JOS antibody diluted at 1:1000.

Immunohistochemistry

IHC of paraffin-embedded H661 xenograft using Ataxin 3 antibody at 1:500 dilution.
IHC of paraffin-embedded H661 xenograft using Ataxin 3 antibody at 1:500 dilution.

Western blot

Sample (50 ug of whole cell lysate). A: mouse brain. 10% SDS PAGE. ATXN3 / JOS antibody diluted at 1:500.
Sample (50 ug of whole cell lysate). A: mouse brain. 10% SDS PAGE. ATXN3 / JOS antibody diluted at 1:500.

Western blot

Sample (30 ug of whole cell lysate). A: U87-MG. 10% SDS PAGE. ATXN3 / JOS antibody diluted at 1:1000.
Sample (30 ug of whole cell lysate). A: U87-MG. 10% SDS PAGE. ATXN3 / JOS antibody diluted at 1:1000.

Immunohistochemistry

IHC of paraffin-embedded H661 xenograft using Ataxin 3 antibody at 1:500 dilution.
IHC of paraffin-embedded H661 xenograft using Ataxin 3 antibody at 1:500 dilution.

Western blot

Sample (50 ug of whole cell lysate). A: mouse brain. 10% SDS PAGE. ATXN3 / JOS antibody diluted at 1:500.
Sample (50 ug of whole cell lysate). A: mouse brain. 10% SDS PAGE. ATXN3 / JOS antibody diluted at 1:500.

Western blot

Sample (30 ug of whole cell lysate). A: U87-MG. 10% SDS PAGE. ATXN3 / JOS antibody diluted at 1:1000.
Sample (30 ug of whole cell lysate). A: U87-MG. 10% SDS PAGE. ATXN3 / JOS antibody diluted at 1:1000.

Immunohistochemistry

IHC of paraffin-embedded H661 xenograft using Ataxin 3 antibody at 1:500 dilution.
IHC of paraffin-embedded H661 xenograft using Ataxin 3 antibody at 1:500 dilution.

Western blot

Sample (50 ug of whole cell lysate). A: mouse brain. 10% SDS PAGE. ATXN3 / JOS antibody diluted at 1:500.
Sample (50 ug of whole cell lysate). A: mouse brain. 10% SDS PAGE. ATXN3 / JOS antibody diluted at 1:500.

Western blot

Sample (30 ug of whole cell lysate). A: U87-MG. 10% SDS PAGE. ATXN3 / JOS antibody diluted at 1:1000.
Sample (30 ug of whole cell lysate). A: U87-MG. 10% SDS PAGE. ATXN3 / JOS antibody diluted at 1:1000.

Requested From: United States
Date Requested: 9/23/2018

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