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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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ATXN3 / JOS Antibody (aa1‑245) LS‑C186388 Best Seller
JOS antibody LS-C186388 is an unconjugated rabbit polyclonal antibody to JOS (ATXN3) from human and mouse. Validated for IHC and WB.
Catalog
Size
Price
LS-C186388-100
100 µl (0.73 mg/ml)
$335
Description
JOS antibody LS-C186388 is an unconjugated rabbit polyclonal antibody to JOS (ATXN3) from human and mouse. Validated for IHC and WB.
Target
Human ATXN3 / JOS
Host
Rabbit
Reactivity
Human, Mouse (tested or 100% immunogen sequence identity)
Predicted
Rat, Bovine, Pig (at least 90% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (1:100 - 1:1000)
  • Western blot (1:500 - 1:3000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
ATXN3 / JOS antibody was raised against recombinant fragment corresponding to a region within amino acids 1 and 245 of Ataxin 3 (SwissProt P54252). Percent identity by BLAST analysis: Human (100%); Pig (99%); Bovine (98%); Rat (96%).
Epitope
aa1-245
Specificity
Human ATXN3 / JOS
Usage
IHC-paraffin: Suggested antigen retrieval using heat mediated 10 mM Citrate buffer (pH 6.0) or Tris-EDTA buffer (pH 8.0).
Presentation
0.1 M Tris-glycine, pH 7.0, 20% glycerol, 0.01% Thimerosal
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Restrictions
For research use only.
About ATXN3 / JOS
P54252 NM_004993 NP_004984.2

Popular ATXN3 / JOS Products

Antibody
Species: Human
Applications: Western blot
Antibody
Species: Human
Applications: Western blot
Antibody
Species: Human
Applications: Western blot
Formalin-fixed and paraffin-embedded human brain tissue reacted with ATXN3 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Species: Human
Applications: IHC, IHC - Paraffin, Western blot, Flow Cytometry

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Images

Immunohistochemistry

IHC of paraffin-embedded H661 xenograft using Ataxin 3 antibody at 1:500 dilution.
IHC of paraffin-embedded H661 xenograft using Ataxin 3 antibody at 1:500 dilution.

Western blot

Sample (50 ug of whole cell lysate). A: mouse brain. 10% SDS PAGE. ATXN3 / JOS antibody diluted at 1:500.
Sample (50 ug of whole cell lysate). A: mouse brain. 10% SDS PAGE. ATXN3 / JOS antibody diluted at 1:500.

Western blot

Sample (30 ug of whole cell lysate). A: U87-MG. 10% SDS PAGE. ATXN3 / JOS antibody diluted at 1:1000.
Sample (30 ug of whole cell lysate). A: U87-MG. 10% SDS PAGE. ATXN3 / JOS antibody diluted at 1:1000.

Immunohistochemistry

IHC of paraffin-embedded H661 xenograft using Ataxin 3 antibody at 1:500 dilution.
IHC of paraffin-embedded H661 xenograft using Ataxin 3 antibody at 1:500 dilution.

Western blot

Sample (50 ug of whole cell lysate). A: mouse brain. 10% SDS PAGE. ATXN3 / JOS antibody diluted at 1:500.
Sample (50 ug of whole cell lysate). A: mouse brain. 10% SDS PAGE. ATXN3 / JOS antibody diluted at 1:500.

Western blot

Sample (30 ug of whole cell lysate). A: U87-MG. 10% SDS PAGE. ATXN3 / JOS antibody diluted at 1:1000.
Sample (30 ug of whole cell lysate). A: U87-MG. 10% SDS PAGE. ATXN3 / JOS antibody diluted at 1:1000.

Immunohistochemistry

IHC of paraffin-embedded H661 xenograft using Ataxin 3 antibody at 1:500 dilution.
IHC of paraffin-embedded H661 xenograft using Ataxin 3 antibody at 1:500 dilution.

Western blot

Sample (50 ug of whole cell lysate). A: mouse brain. 10% SDS PAGE. ATXN3 / JOS antibody diluted at 1:500.
Sample (50 ug of whole cell lysate). A: mouse brain. 10% SDS PAGE. ATXN3 / JOS antibody diluted at 1:500.

Western blot

Sample (30 ug of whole cell lysate). A: U87-MG. 10% SDS PAGE. ATXN3 / JOS antibody diluted at 1:1000.
Sample (30 ug of whole cell lysate). A: U87-MG. 10% SDS PAGE. ATXN3 / JOS antibody diluted at 1:1000.

Immunohistochemistry

IHC of paraffin-embedded H661 xenograft using Ataxin 3 antibody at 1:500 dilution.
IHC of paraffin-embedded H661 xenograft using Ataxin 3 antibody at 1:500 dilution.

Western blot

Sample (50 ug of whole cell lysate). A: mouse brain. 10% SDS PAGE. ATXN3 / JOS antibody diluted at 1:500.
Sample (50 ug of whole cell lysate). A: mouse brain. 10% SDS PAGE. ATXN3 / JOS antibody diluted at 1:500.

Western blot

Sample (30 ug of whole cell lysate). A: U87-MG. 10% SDS PAGE. ATXN3 / JOS antibody diluted at 1:1000.
Sample (30 ug of whole cell lysate). A: U87-MG. 10% SDS PAGE. ATXN3 / JOS antibody diluted at 1:1000.

Requested From: United States
Date Requested: 11/19/2018
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