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Catalog Number Size Price
LS-C288768-10 10 µg $295 
LS-C288768-100 100 µl (1 mg/ml) $533 
ZAP70 Antibody - Flow cytometric analysis of phospho-Zap70 (Y319). Jurkat cells were treated with H2O2 for 3 minutes. Cells were fixed in 1.5% PFA, permeabilized in 90%, and 1 X 10e6 cells stained with 1.0 ug/ml Phospho Zap70 (Y319) antibody in a 150 mcl volume. Isotype anti-KLH control (black) in H2O2 treated cells, anti-phospho Zap70 (Y319) in untreated cells (red), anti-phospho Zap70 (Y319) in H2O2 treated (blue).

Polyclonal Rabbit anti‑Human ZAP70 Antibody (phospho‑Tyr319) LS‑C288768

Polyclonal Rabbit anti‑Human ZAP70 Antibody (phospho‑Tyr319) LS‑C288768

Antibody:
ZAP70 Rabbit anti-Human Polyclonal (pTyr319) Antibody
Application:
IP, Flo
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$295
LS-C288768-10
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
ZAP70 Rabbit anti-Human Polyclonal (pTyr319) Antibody
Application:
IP, Flo
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
ZAP70 antibody LS-C288768 is an unconjugated rabbit polyclonal antibody to human ZAP70 (pTyr319). Validated for Flow and IP.
Target
Human ZAP70
Synonyms
ZAP70 | 70 kDa zeta-associated protein | SRK | STCD | STD | Syk-related tyrosine kinase | Tyrosine-protein kinase ZAP-70 | TZK | ZAP-70 | Zeta-associated protein 70
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Predicted
Mouse (at least 90% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Affinity purified
Modifications
Unmodified
Immunogen
A portion of GeneID: 7535 corresponding to amino acids surrounding tyrosine 319 of UniprotID: P43403
Epitope
pTyr319
Specificity
Region including tyrosine 319 (when it is phosphorylated) of human zeta-chain (TCR) associated protein kinase 70kD using the numbering given in entry NP_001070.2 (GeneID 7535).
Applications
  • Immunoprecipitation (2 - 5 µg/mg lysate)
  • Flow Cytometry
Failed Applications
  • Western blot
Usage
Flo: 1.0 µg per 1 X 10^6 cells in a 150 µl volume
Manufacturer
Bethyl Laboratories, Inc.
Presentation
Tris-citrate/phosphate buffer, pH 7-8, 0.09% sodium azide.
Storage
Store at 2-8°C for up to one year.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About ZAP70
P43403 NM_207519 P43403

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 4/26/2024