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XAF1 Antibody (aa107‑156) LS‑C107929
XAF1 antibody LS-C107929 is an unconjugated rabbit polyclonal antibody to human XAF1. Validated for WB.
Catalog
Size
Price
LS-C107929-50
50 µg
Unavailable

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Product Description

XAF1 antibody LS-C107929 is an unconjugated rabbit polyclonal antibody to human XAF1. Validated for WB.
About XAF1
Seems to function as a negative regulator of members of the IAP (inhibitor of apoptosis protein) family. Inhibits anti-caspase activity of BIRC4. Induces cleavage and inactivation of BIRC4 independent of caspase activation. Mediates TNF-alpha-induced apoptosis and is involved in apoptosis in trophoblast cells. May inhibit BIRC4 indirectly by activating the mitochondrial apoptosis pathway. Q6GPH4 NM_017523 NP_059993.2

XAF1 Antibody, BIRC4 binding protein Antibody, BIRC4-binding protein Antibody, BIRC4BP Antibody, HSXIAPAF1 Antibody, XIAP-associated factor 1 Antibody, XIAPAF1 Antibody, XIAP associated factor 1 Antibody


Specifications

Target
Human XAF1
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Predicted
Monkey (at least 90% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated. Also available conjugated with Biotin, FITC, HRP.
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • Western blot (0.2 - 1 µg/ml)
  • (applications tested for the base form of this product only)
Immunogen
XAF1 antibody was raised against synthetic peptide located between aa107-156 of human XAF1 (Q6GPH4, NP_059993). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla (100%); Monkey (92%); Gibbon (85%).
Blocking Peptide
LS-E20759 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C107929. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa107-156
Specificity
Human XAF1
Usage
Western Blot: Suggested dilution at 1.0µg/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:312500.
Presentation
Lyophilized from PBS with 2% sucrose
Reconstitution
Distilled water
Storage
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Western blot

XAF1 antibody LS-C107929 Western blot of HeLa lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
XAF1 antibody LS-C107929 Western blot of HeLa lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

XAF1 antibody LS-C107929 Western blot of HeLa lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
XAF1 antibody LS-C107929 Western blot of HeLa lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

XAF1 antibody LS-C107929 Western blot of HeLa lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
XAF1 antibody LS-C107929 Western blot of HeLa lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

XAF1 antibody LS-C107929 Western blot of HeLa lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
XAF1 antibody LS-C107929 Western blot of HeLa lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.


Requested From: 
Date Requested: 6/21/2018

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