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Anti-VEGFA / VEGF Antibody LS-C154028

Ordering

Wt. Vol. Conc. Price
100 µg 100 µl - $405
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular VEGFA / VEGF Antibodies

Anti-VEGFA / VEGF Antibody (clone VG76e) LS-C2929
Mouse Monoclonal [clone VG76e] (IgG1) to Human VEGFA / VEGF
Human, Bovine, Pig, Sheep
IHC - Paraffin, Western blot, Immunoprecipitation, ELISA
Unconjugated
Western blot Image
Anti-VEGFA / VEGF Antibody LS-C104581
Rabbit Polyclonal to Human VEGFA / VEGF
Human
IHC, Western blot, ELISA, Neutralization
Unconjugated
Immunohistochemistry Image
Anti-VEGFA / VEGF Antibody (clone VG-1) IHC-plus™ LS-B3579
Mouse Monoclonal [clone VG-1] (IgG1) to Human VEGFA / VEGF
Human
IHC - Paraffin, Western blot, ELISA
Unconjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal (IgG) to Bovine VEGFA / VEGF
Bovine
Western blot, ELISA
Unconjugated

Details

Bovine VEGFA / VEGF
Rabbit
Bovine (tested or 100% immunogen sequence identity)
IgG Polyclonal
Unconjugated
Protein A purified
Unmodified

Applications

  • Western blot (1:333)
  • ELISA

Specificity and Use

VEGFA / VEGF antibody was raised against recombinant protein produced in yeast, corresponding to the 164 amino acids of the mature bovine VEGF-A protein.
This antibody is specific for bovine VEGF-A protein. A BLAST analysis was used to suggest cross-reactivity with VEGF-A from bovine, pig, sheep, and macaque sources based on 100% homology with the immunizing sequence. Partial reactivity is expected against human, horse, dog, cat, or guinea pig based on 95% homology; and to rat and mouse based on 93% homology. Cross-reactivity with VEGF-A from other sources has not been determined.
This protein-A purified antibody has been tested for use in ELISA and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 19.2 kD in size corresponding to bovine VEGF-A protein by western blotting in the appropriate cell lysate or extract.

Packaging

0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.01% sodium azide
100 µl deionized water
Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles.
For research use only.

About VEGFA / VEGF

P15692 NM_003376 NP_003367.4

VEGFA Antibody, VPF Antibody, Vascular permeability factor Antibody, VEGF Antibody, VEGF-A Antibody, MVCD1 Antibody

VEGFA / VEGF is a member of the PDGF/VEGF growth factor family and encodes a protein that is often found as a disulfide linked homodimer. This protein is a glycosylated mitogen that specifically acts on endothelial cells and has various effects, including mediating increased vascular permeability, inducing angiogenesis, vasculogenesis and endothelial cell growth, promoting cell migration, and inhibiting apoptosis.

Western blot

Western blot
Anti-Bovine VEGF-A Antibody - Western Blot. Western blot of Protein-A Purified anti-bovine VEGF-A antibody shows detection of recombinant bovine VEGF-A at 17-19.2 kD (arrowhead). Approximately 2 ug of recombinant protein was loaded per lane onto a 4-20% gradient gel followed by transfer to PVDF membrane. The membrane was blocked using 3% BSA (BSA-30) diluted 1:10. The primary antibody was used at a 1:333 dilution and was incubated with the blot for 2h at room temperature. The membrane was washed and reacted with a 1:10000 dilution of IRDye800 Conjugated Affinity Purified Goat-anti-Rabbit IgG [H&L] MX10. Molecular weight estimation was made by comparison to prestained MW markers. Other detection systems will yield similar results.
 

Requested From: United States
Date Requested: 12/4/2016

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