Bovine VEGFA / VEGF

Rabbit

Bovine (tested or 100% immunogen sequence identity)

Human, Mouse, Rat, Cat, Dog, Guinea pig, Horse, Pig, Sheep, Macaque (at least 90% immunogen sequence identity)

IgG Polyclonal

Unconjugated

Protein A affinity chromatography

Lyophilized

This protein-A purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a recombinant protein raised in yeast, corresponding to the 164 amino acids of the mature bovine VEGF-A protein.

This antibody is specific for bovine VEGF-A protein. A BLAST analysis was used to suggest cross-reactivity with VEGF-A from bovine, pig, sheep, and macaque sources based on 100% homology with the immunizing sequence. Partial reactivity is expected against human, horse, dog, cat, or guinea pig based on 95% homology; and to rat and mouse based on 93% homology. Cross-reactivity with VEGF-A from other sources has not been determined.

• Western blot (1:333)
• ELISA

This protein-A purified antibody has been tested for use in ELISA and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 19.2 kD in size corresponding to bovine VEGF-A protein by western blotting in the appropriate cell lysate or extract.

Lyophilized from 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide

Reconstitute in 100 µL of deionized water.

Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P15692 NCBI: NM_003376 NP_003367.4