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Anti-VAV3 Antibody (aa567-578) IHC-plus™ LS-B3237

Note: This antibody replaces LS-C55663

Ordering

Wt. Vol. Conc. Price
50 µg - 0.5 mg/ml $395
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular VAV3 Antibodies

Anti-VAV3 Antibody (aa567-578) IHC-plus™ LS-B3237
Goat Polyclonal to Human VAV3
Human, Monkey
IHC - Paraffin, Western blot, ELISA
Unconjugated
Immunohistochemistry Image
Anti-VAV3 Antibody (aa398-583, FITC) LS-C302035
Rabbit Polyclonal (IgG) to Human VAV3
Human
Western blot, ELISA
FITC Conjugated
Western blot Image
Anti-VAV3 Antibody (aa183-380, FITC) LS-C305077
Rabbit Polyclonal (IgG) to Human VAV3
Human
Western blot, ELISA
FITC Conjugated
Western blot Image

100% Guaranteed 100% Guaranteed
Goat Polyclonal to Human VAV3
Human, Monkey
IHC - Paraffin, Western blot, ELISA
Unconjugated

Details

Human VAV3
Goat
Human, Monkey (tested or 100% immunogen sequence identity)
Polyclonal
Unconjugated
Immunoaffinity purified
Unmodified

Applications

  • IHC - Paraffin (5 µg/ml)
  • Western blot (0.3 - 1 µg/ml)
  • ELISA (1:128000)

Specificity and Use

VAV3 antibody was raised against synthetic peptide C-SGEQGTLKLPEK from an internal region of human VAV3 (NP_006104.4; NP_001073343.1). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset (100%); Opossum (92%); Elephant, Dog, Rabbit, Pig (83%).
LS-E25761 - Lyophilized - 100 µg - $145.00
aa567-578
Human VAV3. This antibody is expected to recognize both reported isoforms (NP_006104.4; NP_001073343.1).
Immunohistochemistry: LS-B3237 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3237 was determined to be 5 ug/ml.

Packaging

Tris-buffered saline, pH 7.3, 0.5% BSA, 0.02% sodium azide
Store at -20°C. Minimize freezing and thawing.
For research use only.

About VAV3

Q9UKW4 NM_006113 NP_006104.4

VAV3 Antibody, VAV-3 Antibody, Vav 3 oncogene Antibody

Exchange factor for GTP-binding proteins RhoA, RhoG and, to a lesser extent, Rac1. Binds physically to the nucleotide-free states of those GTPases. Plays an important role in angiogenesis. Its recruitment by phosphorylated EPHA2 is critical for EFNA1-induced RAC1 GTPase activation and vascular endothelial cell migration and assembly (By similarity). May be important for integrin-mediated signaling, at least in some cell types.

Immunohistochemistry

Immunohistochemistry
Anti-VAV3 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3237 concentration 5 ug/ml.

Immunohistochemistry

Immunohistochemistry
Anti-VAV3 antibody IHC of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3237 concentration 5 ug/ml.

Western blot

Western blot
Antibody (0.3 ug/ml) staining of human bone marrow lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
 

Requested From: United States
Date Requested: 12/4/2016

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