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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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UBE2K / LIG Antibody (aa144‑157) IHC‑plus™ LS‑B4927
Note: This antibody replaces LS-C54966
LIG antibody LS-B4927 is an unconjugated goat polyclonal antibody to LIG (UBE2K) from human, mouse, rat and other species. Validated for IHC, Peptide-ELISA and WB. Tested on 20 paraffin-embedded human tissues.
Catalog
Size
Price
LS-B4927-50
50 µg (0.5 mg/ml)
Unavailable

Popular UBE2K / LIG Products

Antibody
Species: Human
Applications: Western blot
Anti-UBE2K / LIG antibody IHC staining of human colon. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B10316 concentration 5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Goat, Guinea pig, Hamster, Horse, Pig, Rabbit, Zebrafish
Applications: IHC, IHC - Paraffin, Western blot
Anti-UBE2K / LIG antibody IHC staining of human colon. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B10332 concentration 5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Goat, Guinea pig, Hamster, Horse, Pig, Rabbit, Chicken, Xenopus, Zebrafish
Applications: IHC, IHC - Paraffin, Western blot
Antibody
Species: Human
Applications: IHC, Western blot, ELISA

Product Description

LIG antibody LS-B4927 is an unconjugated goat polyclonal antibody to LIG (UBE2K) from human, mouse, rat and other species. Validated for IHC, Peptide-ELISA and WB. Tested on 20 paraffin-embedded human tissues.
About UBE2K / LIG
Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro, in the presence or in the absence of BRCA1-BARD1 E3 ubiquitin-protein ligase complex, catalyzes the synthesis of 'Lys-48'-linked polyubiquitin chains. Does not transfer ubiquitin directly to but elongates monoubiquitinated substrate protein. P61086 NM_005339 NP_005330.1

UBE2K Antibody, E2-25K Antibody, E2(25K) Antibody, HIP2 Antibody, HYPG Antibody, LIG Antibody, UBC1 Antibody, Ubiquitin-protein ligase Antibody, HIP-2 Antibody, Ubiquitin carrier protein Antibody


Specifications

Target
Human UBE2K / LIG
Host
Goat
Reactivity
Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Goat, Hamster, Horse, Pig, Rabbit, Chicken, Xenopus, Zebrafish (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (3.75 µg/ml)
  • Western blot (1:2000) & (1 - 3 µg/ml)
  • Peptide Enzyme-Linked Immunosorbent Assay (1:2000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
UBE2K / LIG antibody was raised against synthetic peptide C-SWDVETATELLLSN from the C-terminus of human UBE2K / HIP2 (NP_005330.1; NP_001104582.1; NP_001104583.1). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Goat, Hamster, Elephant, Panda, Dog, Bat, Bovine, Horse, Rabbit, Pig, Opossum, Turkey, Chicken, Platypus, Lizard, Xenopus, Salmon, Stickleback, Pufferfish, Zebrafish (100%).
Blocking Peptide
LS-E25469 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-B4927. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa144-157
Specificity
Human UBE2K / HIP2. This antibody is expected to recognise isoform 1 (NP_005330.1), isoform 2 (NP_001104582.1) and isoform 3 (NP_001104583.1).
Presentation
Tris-buffered saline, pH 7.3, 0.5% BSA, 0.02% sodium azide
Storage
Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Immunohistochemistry

Anti-UBE2K / HIP2 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4927 concentration 3.75 ug/ml.
Anti-UBE2K / HIP2 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4927 concentration 3.75 ug/ml.

Immunohistochemistry

Anti-UBE2K / HIP2 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4927 concentration 3.75 ug/ml.
Anti-UBE2K / HIP2 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4927 concentration 3.75 ug/ml.

Western blot

Staining (1.5?g/ml) of Jurkat lysate (RIPA buffer, 35?g total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
Staining (1.5?g/ml) of Jurkat lysate (RIPA buffer, 35?g total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

Western blot

Antibody staining (1.5 ug/ml) of Jurkat lysate (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by Western blot of chemiluminescence.
Antibody staining (1.5 ug/ml) of Jurkat lysate (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by Western blot of chemiluminescence.

Immunohistochemistry

Anti-UBE2K / HIP2 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4927 concentration 3.75 ug/ml.
Anti-UBE2K / HIP2 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4927 concentration 3.75 ug/ml.

Immunohistochemistry

Anti-UBE2K / HIP2 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4927 concentration 3.75 ug/ml.
Anti-UBE2K / HIP2 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4927 concentration 3.75 ug/ml.

Western blot

Staining (1.5?g/ml) of Jurkat lysate (RIPA buffer, 35?g total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
Staining (1.5?g/ml) of Jurkat lysate (RIPA buffer, 35?g total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

Western blot

Antibody staining (1.5 ug/ml) of Jurkat lysate (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by Western blot of chemiluminescence.
Antibody staining (1.5 ug/ml) of Jurkat lysate (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by Western blot of chemiluminescence.

Immunohistochemistry

Anti-UBE2K / HIP2 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4927 concentration 3.75 ug/ml.
Anti-UBE2K / HIP2 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4927 concentration 3.75 ug/ml.

Immunohistochemistry

Anti-UBE2K / HIP2 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4927 concentration 3.75 ug/ml.
Anti-UBE2K / HIP2 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4927 concentration 3.75 ug/ml.

Western blot

Staining (1.5?g/ml) of Jurkat lysate (RIPA buffer, 35?g total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
Staining (1.5?g/ml) of Jurkat lysate (RIPA buffer, 35?g total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

Western blot

Antibody staining (1.5 ug/ml) of Jurkat lysate (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by Western blot of chemiluminescence.
Antibody staining (1.5 ug/ml) of Jurkat lysate (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by Western blot of chemiluminescence.

Immunohistochemistry

Anti-UBE2K / HIP2 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4927 concentration 3.75 ug/ml.
Anti-UBE2K / HIP2 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4927 concentration 3.75 ug/ml.

Immunohistochemistry

Anti-UBE2K / HIP2 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4927 concentration 3.75 ug/ml.
Anti-UBE2K / HIP2 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4927 concentration 3.75 ug/ml.

Western blot

Staining (1.5?g/ml) of Jurkat lysate (RIPA buffer, 35?g total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
Staining (1.5?g/ml) of Jurkat lysate (RIPA buffer, 35?g total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

Western blot

Antibody staining (1.5 ug/ml) of Jurkat lysate (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by Western blot of chemiluminescence.
Antibody staining (1.5 ug/ml) of Jurkat lysate (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by Western blot of chemiluminescence.


Requested From: 
Date Requested: 6/21/2018

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