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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

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UBA1 / UBE1 Antibody (N‑Terminus) LS‑C112230
UBE1 antibody LS-C112230 is an unconjugated rabbit polyclonal antibody to UBE1 (UBA1) from human, mouse, rat and other species. Validated for WB.
Catalog
Size
Price
LS-C112230-50
50 µg
Unavailable

Popular UBA1 / UBE1 Products

Anti-Ubiquitin Activating Enzyme Antibody - Immunohistochemistry. Affinity Purified anti-Ubiquitin Activating Enzyme antibody was used at a 10 ug/ml to detect UBE1 in a variety of tissues including adrenal, breast, colon (epithelium), kidney, liver, lung (respiratory epithelium), ovary (oocyte and endothelium), pancreas (islet and exocrine), placenta, prostate (epithelium), skin (epithelium), spleen (lymphocytes), stomach (chief), testis, thymus, tonsil, and uterus (glandular, stroma). In many cells a punctate nuclear staining was observed. Other cells showed both cytoplasmic and nuclear staining. This image shows UBE1 staining of human lung tissue. Tissue was formalin-fixed and paraffin embedded. Personal Communi-cation, Tina Roush, LifeSpanBiosciences, Seattle, WA.
Species: Human
Applications: IHC, IHC - Paraffin, Western blot, ELISA
Antibody
Species: Human, Mouse, Rat
Applications: IHC, IHC - Paraffin, ICC, Immunofluorescence, Western blot, Immunoprecipitation, Flow Cytometry, ELISA
Anti-UBA1 / UBE1 antibody IHC staining of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Species: Human
Applications: IHC, IHC - Paraffin, Immunofluorescence
Human Breast: Formalin-Fixed, Paraffin-Embedded (FFPE)
Species: Human, Mouse, Rat, Guinea pig, Rabbit
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot, Immunoprecipitation
Human Breast: Formalin-Fixed, Paraffin-Embedded (FFPE)
Species: Human, Mouse, Rat, Bovine, Guinea pig, Horse, Pig, Rabbit
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot

Product Description

UBE1 antibody LS-C112230 is an unconjugated rabbit polyclonal antibody to UBE1 (UBA1) from human, mouse, rat and other species. Validated for WB.
About UBA1 / UBE1
Ubiquitin Activating Enzyme (E1), also known as A1S9 and UBE1, is responsible for the first step in ubiquitin-protein isopeptide bond formation. E1 catalyzes the activation of the Cterminal carboxyl group of ubiquitin by forming a high-energy thioester bond in an ATP-dependent manner. P22314 NM_003334 NP_003325.2

UBA1 Antibody, A1S9 Antibody, A1ST Antibody, A1S9T Antibody, AMCX1 Antibody, GXP1 Antibody, Protein A1S9 Antibody, SMAX2 Antibody, Ubiquitin-activating enzyme E1 Antibody, UBE1X Antibody, POC20 Antibody, UBE1 Antibody, UBA1A Antibody


Specifications

Target
Human UBA1 / UBE1
Host
Rabbit
Reactivity
Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Guinea pig, Hamster, Horse, Pig, Rabbit (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated. Also available conjugated with Biotin, FITC, HRP.
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • Western blot (0.2 - 1 µg/ml)
  • (applications tested for the base form of this product only)
Immunogen
UBA1 / UBE1 antibody was raised against synthetic peptide from N-Terminus of human UBA1 (P22314, NP_003325). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Galago, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Dog, Bovine, Bat, Rabbit, Horse, Pig, Opossum, Guinea pig (100%).
Blocking Peptide
LS-E21477 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C112230. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
N-Terminus
Specificity
Human UBA1 / UBE1
Usage
Western Blot: Suggested dilution at 1 ug/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.
Presentation
Lyophilized from PBS with 2% sucrose
Reconstitution
Distilled water
Storage
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Western blot

UBA1 / UBE1 antibody LS-C112230 Western blot of HepG2 cell lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
UBA1 / UBE1 antibody LS-C112230 Western blot of HepG2 cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

UBA1 / UBE1 antibody LS-C112230 Western blot of HepG2 cell lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
UBA1 / UBE1 antibody LS-C112230 Western blot of HepG2 cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

UBA1 / UBE1 antibody LS-C112230 Western blot of HepG2 cell lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
UBA1 / UBE1 antibody LS-C112230 Western blot of HepG2 cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

UBA1 / UBE1 antibody LS-C112230 Western blot of HepG2 cell lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
UBA1 / UBE1 antibody LS-C112230 Western blot of HepG2 cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.


Requested From: 
Date Requested: 6/21/2018

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