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Catalog Number Size Price
LS-C18912-500 500 µg $506 
UBA1 / UBE1 Antibody - Anti-Ubiquitin Activating Enzyme Antibody - Immunohistochemistry. Affinity Purified anti-Ubiquitin Activating Enzyme antibody was used at a 10 ug/ml to detect UBE1 in a variety of tissues including adrenal, breast, colon (epithelium), kidney, liver, lung (respiratory epithelium), ovary (oocyte and endothelium), pancreas (islet and exocrine), placenta, prostate (epithelium), skin (epithelium), spleen (lymphocytes), stomach (chief), testis, thymus, tonsil, and uterus (glandular, stroma). In many cells a punctate nuclear staining was observed. Other cells showed both cytoplasmic and nuclear staining. This image shows UBE1 staining of human lung tissue. Tissue was formalin-fixed and paraffin embedded. Personal Communi-cation, Tina Roush, LifeSpanBiosciences, Seattle, WA.
UBA1 / UBE1 Antibody - Anti-Ubiquitin Activating Enzyme (E1) Antibody - Western Blot. Western blot of purified anti-Ubiquitin Activating Enzyme (E1) antibody shows detection of a band at ~118 kD corres-ponding to UBE1 (lane 1 800 nm channel). Approximately 35 ug of an A431 whole cell lysate was separated on a 4-20% Tris-Glycine gel by SDS-PAGE and transferred onto nitrocellulose. After blocking the membrane was probed with the primary antibody diluted to 1:1000. Incubation was for 2 h at room temperature followed by washes and reaction with a 1:10000 dilution of IRDye800 conjugated Gt-a-Rabbit IgG [H&L] MX10 ( for 45 min at room temperature. Molecular weight markers are shown in lane 2 (700 nm channel). IRDye800 fluorescence image was captured using the Odyssey Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.
UBA1 / UBE1 Antibody - Anti-Ubiquitin Activating Enzyme Antibody - Immunohistochemistry. Affinity Purified anti-Ubiquitin Activating Enzyme antibody was used at a 10 ug/ml to detect UBE1 in a variety of tissues including adrenal, breast, colon (epithelium), kidney, liver, lung (respiratory epithelium), ovary (oocyte and endothelium), pancreas (islet and exocrine), placenta, prostate (epithelium), skin (epithelium), spleen (lymphocytes), stomach (chief), testis, thymus, tonsil, and uterus (glandular, stroma). In many cells a punctate nuclear staining was observed. Other cells showed both cytoplasmic and nuclear staining. This image shows UBE1 staining of human lung tissue. Tissue was formalin-fixed and paraffin embedded. Personal Communi-cation, Tina Roush, LifeSpanBiosciences, Seattle, WA.
UBA1 / UBE1 Antibody - Anti-Ubiquitin Activating Enzyme (E1) Antibody - Western Blot. Western blot of purified anti-Ubiquitin Activating Enzyme (E1) antibody shows detection of a band at ~118 kD corres-ponding to UBE1 (lane 1 800 nm channel). Approximately 35 ug of an A431 whole cell lysate was separated on a 4-20% Tris-Glycine gel by SDS-PAGE and transferred onto nitrocellulose. After blocking the membrane was probed with the primary antibody diluted to 1:1000. Incubation was for 2 h at room temperature followed by washes and reaction with a 1:10000 dilution of IRDye800 conjugated Gt-a-Rabbit IgG [H&L] MX10 ( for 45 min at room temperature. Molecular weight markers are shown in lane 2 (700 nm channel). IRDye800 fluorescence image was captured using the Odyssey Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.
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Polyclonal Rabbit anti‑Human UBA1 / UBE1 Antibody (IHC, WB) LS‑C18912

Polyclonal Rabbit anti‑Human UBA1 / UBE1 Antibody (IHC, WB) LS‑C18912

Antibody:
UBA1 / UBE1 Rabbit anti-Human Polyclonal Antibody
Application:
IHC, IHC-P, WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$506
LS-C18912-500
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
UBA1 / UBE1 Rabbit anti-Human Polyclonal Antibody
Application:
IHC, IHC-P, WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
UBE1 antibody LS-C18912 is an unconjugated rabbit polyclonal antibody to human UBE1 (UBA1). Validated for ELISA, IHC and WB.
Target
Human UBA1 / UBE1
Synonyms
UBA1 | A1S9 | A1ST | A1S9T | AMCX1 | GXP1 | Protein A1S9 | SMAX2 | Ubiquitin-activating enzyme E1 | UBE1X | POC20 | UBE1 | UBA1A
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Protein A purified
Modifications
Unmodified
Immunogen
Anti-Ubiquitin Activating Enzyme E1 antibody was prepared from whole rabbit serum produced by repeated immunizations with a recombinant protein corresponding to full length Human Ubiquitin Activating Enzyme E1.
Applications
  • IHC
  • IHC - Paraffin (2 - 20 µg/ml)
  • Western blot (1:1000 - 1:5000)
  • ELISA (1:2000 - 1:10000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
This purified antibody has been tested for use in ELISA, immunohistochemistry and western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band at ~118 kD in size corresponding to UBE1 by western blotting in the appropriate cell lysate or extract.
Presentation
Lyophilized from 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Reconstitution
Reconstitute in 500 µL of deionized water.
Storage
Store vial at 4°C prior to restoration. Reconstitute with 0.1 ml of deionized water or equivalent. For extended storage aliquot contents and freeze at -20°C or below. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About UBA1 / UBE1
P22314 NM_003334 NP_003325.2

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 8/18/2022