(tested or 100% immunogen sequence identity)
- IHC - Paraffin (2 - 20 µg/ml)
- Western blot (1:1000 - 1:5000)
- ELISA (1:2000 - 1:10000)
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UBA1 / UBE1 antibody was raised against recombinant human UBA1 / UBE1.
Full length Human Ubiquitin Activating Enzyme E1 fused with GST.
This purified antibody has been tested for use in ELISA, immunohistochemistry and western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band at ~118 kD in size corresponding to UBE1 by western blotting in the appropriate cell lysate or extract.
After adding water, will consist of 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.01% (w/v) Sodium Azide
Store vial at 4°C prior to restoration. Reconstitute with 0.1 ml of deionized water or equivalent. For extended storage aliquot contents and freeze at -20°C or below. Avoid freeze-thaw cycles.
For research use only.
This antibody carries the LSBio 100% Guarantee.