Target
Human TP53BP1 / 53BP1
Synonyms
TP53BP1 | 53BP1 | p202 | p53-binding protein 1 | p53-binding protein 53bp1 | p53BP1
Reactivity
Human
(tested or 100% immunogen sequence identity)
Clonality
IgG1
Monoclonal
Conjugations
Unconjugated.
Also available conjugated with
APC,
Biotin,
FITC,
HRP,
PE.
Purification
Protein G purified
Immunogen
Mix of three GST fusion proteins corresponding to residues 1-337, 338-671, and 1331-1664, respectively, of human 53BP1.
Specificity
Recognizes human 53BP1, Mr ~250kD.
Applications
- ICC
- Western blot
- Immunoprecipitation
Usage
Suitable for use in Western Blot, Immunoprecipitation and Immunocytochemistry. Western Blot Analysis: 1-4 ug/ml of this lot detected 53BP1 in lysates from HeLa cells. Immunoprecipitation: Has been reported by an independent laboratory to immunoprecipitate 53BP1 from HeLa cell lysate. Immunocytochemistry: Has been reported by an independent laboratory to show positive immunostaining for 53BP1 in HeLa cells fixed with 3% paraformaldehyde and permeabilized in 0.5% Triton X-100.
Presentation
0.1 M Tris-Glycine, pH 7.4, 0.15 M NaCl, 0.05% Sodium Azide, 30% Glycerol
Storage
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee