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Immunohistochemistry Services

Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

TCR Screening Services

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Cell Cycle Pathways
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Catalog Number Size Price
LS-B315-100 100 µl (1 mg/ml) $485 
TP53BP1 / 53BP1 Antibody - Anti-TP53BP1 / 53BP1 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.

IHCPlus™ Polyclonal Rabbit anti‑Human TP53BP1 / 53BP1 Antibody (IHC, WB) LS‑B315

IHCPlus™ Polyclonal Rabbit anti‑Human TP53BP1 / 53BP1 Antibody (IHC, WB) LS‑B315

Note: This antibody replaces LS-C2590
Antibody:
Rabbit Polyclonal to Human TP53BP1 / 53BP1
Application:
IHC, IHC-P, ICC, WB
Reactivity:
Human, Mouse
Format:
Unconjugated, Unmodified
Other formats:
Price
Catalog Number
$485 
LS-B315-100
100 µl
1 mg/ml
Toll Free North America
866-819-4732
For Research Use Only

Overview

Antibody:
Rabbit Polyclonal to Human TP53BP1 / 53BP1
Application:
IHC, IHC-P, ICC, WB
Reactivity:
Human, Mouse
Format:
Unconjugated, Unmodified
Other formats:

Specifications

Description
53BP1 antibody LS-B315 is an unconjugated rabbit polyclonal antibody to 53BP1 (TP53BP1) from human. It is reactive with human and mouse. Validated for ICC, IHC and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human TP53BP1 / 53BP1
Synonyms
TP53BP1 | 53BP1 | p202 | p53-binding protein 1 | p53-binding protein 53bp1 | p53BP1
Host
Rabbit
Reactivity
Human, Mouse (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated. Also available conjugated with Biotin, DY650, DY488, DY550.
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (5 µg/ml)
  • ICC (1:100 - 1:1000)
  • Western blot (1:1000 - 1:10000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
A synthetic peptide, which represented a portion of human tumor protein p53-binding protein 1 encoded in exon 11, 12 and 19 (LocusLink ID 7158).
Specificity
human 53BP1 protein.
Usage
Immunohistochemistry: LS-B315 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B315 was determined to be 5 ug/ml.
Presentation
Tris-citrate/phosphate, pH 7-8, 0.1% sodium azide
Storage
Store at 4°C. Avoid freezing.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About TP53BP1 / 53BP1
Q12888 NM_005657 NP_005648.1

Validation

TP53BP1 / 53BP1 Antibody - Anti-TP53BP1 / 53BP1 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-TP53BP1 / 53BP1 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-TP53BP1 / 53BP1 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

LSBio Ratings

IHC-plus™ TP53BP1 / 53BP1 Antibody for IHC, ICC, WB/Western LS-B315 has an LSBio Rating of
Laboratory Validation Score (4)

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Requested From: United States
Date Requested: 7/3/2020