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IHCPlus™ TP53BP1 / 53BP1 Antibody Polyclonal ICC,IHC,WB LS‑B315

Catalog Number Size Price
LS-B315-100 100 µl (1 mg/ml) $485 
Anti-TP53BP1 / 53BP1 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.

IHCPlus™ TP53BP1 / 53BP1 Antibody Polyclonal ICC,IHC,WB LS‑B315

Note: This antibody replaces LS-C2590
Antibody:
Rabbit Polyclonal to Human TP53BP1 / 53BP1
Application:
IHC, IHC-P, ICC, WB
Reactivity:
Human, Mouse
Format:
Unconjugated, Unmodified
Other formats:
Price
Catalog Number
$485 
LS-B315-100
Toll Free North America
866-819-4732

Overview

Antibody:
Rabbit Polyclonal to Human TP53BP1 / 53BP1
Application:
IHC, IHC-P, ICC, WB
Reactivity:
Human, Mouse
Format:
Unconjugated, Unmodified
Other formats:

Specifications

Description
53BP1 antibody LS-B315 is an unconjugated rabbit polyclonal antibody to 53BP1 (TP53BP1 ) from human and mouse. Validated for ICC, IHC and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human TP53BP1 / 53BP1
Host
Rabbit
Reactivity
Human, Mouse (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated. Also available conjugated with Biotin, DY650, DY488, DY550.
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (5 µg/ml)
  • ICC (1:100 - 1:1000)
  • Western blot (1:1000 - 1:10000)
  • (applications tested for the base form of this product only)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
A synthetic peptide, which represented a portion of human tumor protein p53-binding protein 1 encoded in exon 11, 12 and 19 (LocusLink ID 7158).
Specificity
human 53BP1 protein.
Usage
Immunohistochemistry: LS-B315 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B315 was determined to be 5 ug/ml.
Presentation
Tris-citrate/phosphate, pH 7-8, 0.1% sodium azide
Storage
Store at 4°C. Avoid freezing.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About TP53BP1 / 53BP1
Q12888 NM_005657 NP_005648.1

Validation

Anti-TP53BP1 / 53BP1 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
IHC Validated
Anti-TP53BP1 / 53BP1 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-TP53BP1 / 53BP1 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

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Requested From: United States
Date Requested: 9/17/2019