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Anti-TACSTD1 / EPCAM Antibody (clone MOC-31) IHC-plus™ LS-B5565

Note: This antibody replaces LS-C134852


Wt. Vol. Conc. Price
- 50 µl - $395
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)

Most Popular TACSTD1 / EPCAM Antibodies

Anti-TACSTD1 / EPCAM Antibody (clone VU-1D9) IHC-plus™ LS-B4511
Mouse Monoclonal [clone VU-1D9] (IgG1) to Human TACSTD1 / EPCAM
IHC - Paraffin, IHC - Frozen, ICC, Western blot, Immunoprecipitation, Flow Cytometry
Immunohistochemistry Image
Anti-TACSTD1 / EPCAM Antibody LS-C65470
Mouse Monoclonal (IgG1) to Human TACSTD1 / EPCAM
IHC - Paraffin, ELISA

100% Guaranteed 100% Guaranteed
Mouse Monoclonal [clone MOC-31] (IgG1) to Human TACSTD1 / EPCAM
IHC - Paraffin, IHC - Frozen, Western blot


Human (tested or 100% immunogen sequence identity)
IgG1 Monoclonal [MOC-31]
Tissue culture supernatant


  • IHC - Paraffin (1:100)
  • IHC - Frozen
  • Western blot

Specificity and Use

This antibody is reactive with lung cancer associated antigens and has been studied and categorized in different clusters of reactivity patterns during the First International Workshop on Small Cell Lung Cancer Antigens held in London in April 1987. MOC-31 reacts with most epithelia, and, in lung cancer, all lung carcinomas. The membrane-associated proteins detected by MOC-31 appear to have an apparent molecular weight of 35-40 kD. MOC-31 is not reactive with normal and malignant mesothelia and therefore is especially useful for the detection of carcinoma cells in ascites or pleural effusions (4).
The antibody is useful in immunohistochemistry and immunoblotting. MOC-31 react with antigens detectable in cryostat section. For use on frozen tissue and paraffin tissue after antigen retrieval. Using the antigen retrieval techniques this antibody discriminates between cells which originate from mesothelium and epithelium. General procedure to perform cryostat sectioning and immunostaining: Cryostat sectioning and immunostaining is done as described by de Leij et al. Sections (about 6 micron thick) are cut in a cryostat at -20°C and placed on glass slides. After drying at RT and fixation in acetone (water-free), the sections are washed 2-3 times in PBS. Subsequently (after drying the glass area around the sections) 25 ul of undiluted monoclonal antibody preparation is applied to the wet sections. After incubation for 45 mins. in a humidified atmosphere, the sections are washed again in PBS (2-3 times) and the second step reagent (an appropriately diluted HRPO-conjugated, anti mouse Ig preparation, supplemented with 1% human serum) is applied to the wet sections. After incubation for an additional 20 mins., the sections are washed again in PBS (2-3) times and the staining reaction is performed with 3-aminoethylcarbazole (10 mg dissolved in 2.5 ml dimethylformamide, subsequently 0.05 M acetate buffer, pH 4.9, is added to a total volume of 50 ml, after which the solution is filtrated and H2O2 is added to a final concentration of about 0.03%). A positive reaction is indicated by red deposit. The nuclei of the cells present in the sections are counterstained with Mayer's hematoxylin to obtain a good histological picture. Working dilution: approx 1:20.


Tissue culture supernatant (8% FBS), <0.1% sodium azide
+4°C. Store undiluted.
For research use only.


P16422 NM_002354 NP_002345.2

EPCAM Antibody, 323/A3 Antibody, ACSTD1 Antibody, 17-1A Antibody, CD326 Antibody, EGP Antibody, EGP34 Antibody, Epithelial glycoprotein Antibody, GA733-2 Antibody, HNPCC8 Antibody, Ep-CAM Antibody, ESA Antibody, HEGP314 Antibody, KS 1/4 antigen Antibody, KS1/4 Antibody, KSA Antibody, M1S2 Antibody, MIC18 Antibody, MK-1 Antibody, MH99 Antibody, TROP1 Antibody, TACST-1 Antibody, TACSTD1 Antibody, CD326 antigen Antibody, CO-17A Antibody, DIAR5 Antibody, EGP-2 Antibody, EGP314 Antibody, EGP40 Antibody, Epithelial glycoprotein 314 Antibody, HEA125 Antibody, Ly74 Antibody, M4S1 Antibody, MOC31 Antibody

May act as a physical homophilic interaction molecule between intestinal epithelial cells (IECs) and intraepithelial lymphocytes (IELs) at the mucosal epithelium for providing immunological barrier as a first line of defense against mucosal infection. Plays a role in embryonic stem cells proliferation and differentiation. Up-regulates the expression of FABP5, MYC and cyclins A and E.


Anti-EPCAM antibody IHC of human intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B5565 dilution 1:100.


Anti-EPCAM antibody IHC of human uterus, endometrium. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B5565 dilution 1:100.

Requested From: United States
Date Requested: 10/26/2016

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