Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
SUMO1 Antibody, GAP modifying protein 1 Antibody, GMP1 Antibody, OFC10 Antibody, Sentrin Antibody, SMT3 homolog 3 Antibody, SMT3C Antibody, SMT3H3 Antibody, SUMO-1 Antibody, Ubiquitin-like protein UBL1 Antibody, PIC1 Antibody, Ubiquitin-like 1 (sentrin) Antibody, UBL1 Antibody, DAP1 Antibody, GAP-modifying protein 1 Antibody, Ubiquitin-like protein SMT3C Antibody
Ubiquitin-like protein that can be covalently attached to proteins as a monomer or a lysine-linked polymer. Covalent attachment via an isopeptide bond to its substrates requires prior activation by the E1 complex SAE1-SAE2 and linkage to the E2 enzyme UBE2I, and can be promoted by E3 ligases such as PIAS1-4, RANBP2 or CBX4.
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
Immunohistochemical of paraffin-embedded H.bladder section using Pan SUMO Antibody. Antibody was diluted at 1:100 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
Fluorescent confocal image of HeLa cell stained with Pan SUMO Antibody. HeLa cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with Pan SUMO primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°C). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°C). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). Pan SUMO immunoreactivity is localized to Nucleus significantly.
SUMO3 Antibody western blot of 293 cell line and rat liver tissue lysates (35 ug/lane). The SUMO3 antibody detected the SUMO3 protein (arrow).