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Anti-SUMO1 / SMT3 Antibody LS-C98837

Ordering

Wt. Vol. Conc. Price
- 400 µl - $305
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular SUMO1 / SMT3 Antibodies

Anti-SUMO1 / SMT3 Antibody (N-Terminus) IHC-plus™ LS-B594
Rabbit Polyclonal to Human SUMO1 / SMT3
Human, Mouse, Rat
IHC - Paraffin, Western blot
Unconjugated
Immunohistochemistry Image
Anti-SUMO1 / SMT3 Antibody (clone 5B12) LS-C179834
Mouse Monoclonal [clone 5B12] (IgG1) to Human SUMO1 / SMT3
Human, Mouse, Rat
ICC, Western blot
Unconjugated
Western blot Image
Anti-SUMO1 / SMT3 Antibody (Biotin) LS-C282588
Mouse Monoclonal (IgG1) to Human SUMO1 / SMT3
Human
Western blot, ELISA
Biotin Conjugated

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal to Human SUMO1 / SMT3
Human
IHC - Paraffin, Immunofluorescence, Western blot
Unconjugated

Details

Human SUMO1 / SMT3
Rabbit
Human (tested or 100% immunogen sequence identity)
Polyclonal
Unconjugated
Ammonium sulfate precipitation
Unmodified

Applications

  • IHC - Paraffin (1:50 - 1:100)
  • Immunofluorescence (1:10 - 1:50)
  • Western blot (1:1000)

Specificity and Use

SUMO1 / SMT3 antibody was raised against recombinant protein encoding full length human SUMO3.".

Packaging

PBS, 0.09% sodium azide
Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.
For research use only.

About SUMO1 / SMT3

P63165 NM_003352 NP_003343.1

SUMO1 Antibody, GAP modifying protein 1 Antibody, GMP1 Antibody, OFC10 Antibody, Sentrin Antibody, SMT3 homolog 3 Antibody, SMT3C Antibody, SMT3H3 Antibody, SUMO-1 Antibody, Ubiquitin-like protein UBL1 Antibody, PIC1 Antibody, Ubiquitin-like 1 (sentrin) Antibody, UBL1 Antibody, DAP1 Antibody, GAP-modifying protein 1 Antibody, Ubiquitin-like protein SMT3C Antibody

Ubiquitin-like protein that can be covalently attached to proteins as a monomer or a lysine-linked polymer. Covalent attachment via an isopeptide bond to its substrates requires prior activation by the E1 complex SAE1-SAE2 and linkage to the E2 enzyme UBE2I, and can be promoted by E3 ligases such as PIAS1-4, RANBP2 or CBX4.

Immunohistochemistry

Immunohistochemistry
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.

Immunohistochemistry

Immunohistochemistry
Immunohistochemical of paraffin-embedded H.bladder section using Pan SUMO Antibody. Antibody was diluted at 1:100 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.

Immunofluorescence

Immunofluorescence
Fluorescent confocal image of HeLa cell stained with Pan SUMO Antibody. HeLa cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with Pan SUMO primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°C). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°C). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). Pan SUMO immunoreactivity is localized to Nucleus significantly.

Western blot

Western blot
SUMO3 Antibody western blot of 293 cell line and rat liver tissue lysates (35 ug/lane). The SUMO3 antibody detected the SUMO3 protein (arrow).

Requested From: United States
Date Requested: 12/10/2016

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