Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Transition from a nucleosome-based to a protamine-based chromatin configuration during spermiogenesis in Drosophila. Rathke C, Baarends WM, Jayaramaiah-Raja S, Bartkuhn M, Renkawitz R, Renkawitz-Pohl R. Journal of cell science. 2007 120:1689-700.
SUMO1 Antibody, GAP modifying protein 1 Antibody, GMP1 Antibody, OFC10 Antibody, Sentrin Antibody, SMT3 homolog 3 Antibody, SMT3C Antibody, SMT3H3 Antibody, SUMO-1 Antibody, Ubiquitin-like protein UBL1 Antibody, PIC1 Antibody, Ubiquitin-like 1 (sentrin) Antibody, UBL1 Antibody, DAP1 Antibody, GAP-modifying protein 1 Antibody, Ubiquitin-like protein SMT3C Antibody
Ubiquitin-like protein that can be covalently attached to proteins as a monomer or a lysine-linked polymer. Covalent attachment via an isopeptide bond to its substrates requires prior activation by the E1 complex SAE1-SAE2 and linkage to the E2 enzyme UBE2I, and can be promoted by E3 ligases such as PIAS1-4, RANBP2 or CBX4.
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
COS-7 cells were transfected for 24 hrs with a plasmid expressing FLAG-ERM (left panels) or FLAG-ERM KR12345 (right panels). Untreated (-) and H2O2-treated (+) cells were collected for immunoblot analysis. Top panels: cell lysates probed by western blot (WB) with an anti-ERM antibody. Center panels: cell lysates immunoprecipitated (IP) with an anti-FLAG antibody followed by WB SUMO-1 antibody. Bottom panels: cell lysates immunoprecipitated with an anti-FLAG antibody followed by WB with SUMO-2/3 antibody. (*) represents immunoprecipitated ERM-like forms recognized by anti-SUMO antibodies.
The anti-SUMO1 polyclonal antibody is used in Western blot to detect GST-SUMO1 fusion protein.
SUMO1 Antibody flow cytometry of HeLa cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Requested From: United States
Date Requested: 1/24/2017