Target
E. coli Sucrose Phosphorylase
Reactivity
E. coli
(tested or 100% immunogen sequence identity)
Conjugations
Unconjugated
Purification
Ion exchange chromatography
Immunogen
Sucrose Phosphorylase (E. coli).
Specificity
Assay by IEP resulted in a single precipitin arc against anti-Goat Serum as well as purified and partially purified Sucrose Phosphorylase.
Applications
- Western blot
- Immunoprecipitation
- ELISA (1:6000 - 1:30000)
Usage
Suitable for Western Blot (Western or Dot Blot), ELISA, Immunoprecipitation, Conjugation and. most immunological methods. ELISA: 1:6000-1:30000; 1 ug of Sucrose Phosphorylase was used in a standard sandwich ELISA with Rabbit anti-Goat (HRP) and ABTS, as a substrate for 30 minutes at RT.
Presentation
Lyophilized from PBS, pH 7.2, 0.01% Sodium Azide
Reconstitution
Reconstitute with 100 µl sterile water.
Storage
Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee